胶糖

The results indicated that DNase E belonging to endonuclease.Conclusion1. Established a preparation technics of rude extracts of Lumbricus Bimastus nucleases.

应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，证明了DNase E属于核酸内切酶。

A preliminary study on substrate specificity of Lumbricus Bimastus nucleaseTo measure degrade rate of DNase E to several different substrate in optimal reaction condition to demonstrate the substrate selectivity of DNase E. Agarose gel electrophoresis was used to investigate the products of DNase E processing double strands cyclic DNA to investigate the action of DNase E.

双胸蚓组织核酸酶底物特异性的研究测定DNase E在最适反应条件下对不同底物的降解速率，证明DNase E降解底物的选择性；应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，阐明DNase E的水解方式。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

Methods To collect 10 specimen residual ear cartilage of microtia patients about 8-year-old and cultured in vitro, dye with toluidine blue and immunohistochemistry of collagen type II.

结果：残耳软骨细胞在体外培养时随代数增加呈异染陛的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng／mi浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

Methods Based on molecular assembly technique, the matrix of agarose gel was deposited on the surface of glass plates,followed by drying, oxidation with NaIO 4 and cross linking with glutaraldehyde.

利用分子组装技术，以琼脂糖凝胶为基质，于玻片表面制成自组装膜，经过碘酸钠氧化后再与戊二醛分子偶联，而成为具有双功能分子层的薄膜。

Nither CDMP-1, nor cryopreservation can effect cell cycle of the chondrocytes.

结果：残耳软骨细胞在体外培养时随代数增加呈异染性的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％/～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng/ml浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

Mutant mice than in wild-type controls, and tetrodotoxin or bicuculline application abolished the difference between the genotypes. Studies on human oligodendroglioma cells revealed that GABA played a protective role on Ca〓 overload induced by OGD or glutamate and phaclofen, GABA〓 receptor antagonist, could significantly block the protection.

结果表明：①电凝大脑中动脉后Ca〓2.3-／-鼠脑梗死率和缺血后运动障碍明显重于Ca〓2.3+／+鼠；②Ca〓2.3-／-鼠海马脑片CA1区缺氧后钙超载明显高于Ca〓2.3+／+鼠；③Na〓通道阻滞剂TTX和GABA〓受体阻断剂bicuculline可显著缩小遗传表型之间的差异；④GABA对缺氧无糖及谷氨酸所致的人胶质细胞瘤细胞Ca〓超载有明显的保护作用，此作用可被GABA〓受体阻断剂phaclofen拮抗。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后，通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。