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The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带,用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

Thus,CMG-KALA nanoparticles have great potential as gene vectors for transfection in vitro.2.GS-peptide nanoparticles were synthesized as previous reported method in order to improve the ability of cell penetrating,endosome escaping and nuclear targeting.

通过激光共聚焦、荧光光谱以及红外光谱证明KALA可以成功地连接到CMG纳米颗粒表面;通过静电引力吸附将DNA与CMG-KALA纳米颗粒进行复合,并通过琼脂糖凝胶电泳证明该纳米颗粒对DNA具有很好的保护作用。

Objective To investigate the effect of glycation of collagen Ⅳ on biological character of epidermal stem cells.

目的 研究糖尿病患者皮肤组织中Ⅳ型胶原的糖基化对表皮干细胞生物学特性的影响。

To investigate the physiological importance of particular vWF domains, we have introduced mutations into murine vWF cDNA inhibiting vWF binding to glycoprotein Ib, GpIIbIIIa, and to fibrillar collagen.

为研究vWF特定区域的生理作用,我们在鼠vWF的cDNA上引入变异株,抑制vWF与糖蛋白lb、GpIIbIIIa以及纤维胶原结合。

RESULTS: Chitosan could significantly inhibit fibroblastic proliferation and type Ⅲ procollagen mRNA expression and showed dose-dependent.

结果:几丁糖能明显抑制成纤维细胞增殖及Ⅲ型前胶原mRNA的表达,并呈剂量依赖性。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后,加入不同浓度的碘化钾,观察细胞的形态改变;琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解;MDA法测定细胞膜的脂质过氧化程度。

Meanwhile, molecular weight of pectin was related to its physicochemical properties. The larger the molecular weight of pectin was, the more its galacturonic acid content and degree of esterification and gelatination were.

相对分子质量与其理化性质有着对应的关系,果胶半乳糖醛酸含量、酯化度和胶凝度随相对分子质量增大而增大,单糖组分也随相对分子质量增大而增多。

Effects of several gelatinizers on low sugar jam were discussed and the best gelatinizer was defined.

以红枣为主要原料,通过改变各辅料的添加量,用正交试验设计筛选出红枣低糖果酱的最佳配方,并对几种增稠剂的增稠效果和凝胶效果进行了探讨,确定了最佳增稠剂及其用量。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞,用不同浓度的HCY伴/不伴生理浓度Cu~(2+)与内皮细胞作用;细胞计数板检测脱落细胞率;MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤;Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡,并采用流式细胞术定量测定细胞凋亡率。

Then the tissue and blood samples were obtained and measured.Result: In the muscovite group,restored mucosa thickness increased,cystically dilated glands decreased,microvessels in connective tissue increased,the secretion of mucus,hexosamine, PGE2,EGF,bFGF were enhanced,and the express of EGFR was stronger.

结果:经云母治疗后,大鼠再生黏膜厚度增加、囊状扩张腺体数目减少,肉芽组织炎症细胞浸润数量减少、微血管数量增加、胶原面积增加,黏膜中性黏液和氨基己糖分泌增多,血浆PGE2,EGF分泌增多,免疫组化染色中黏膜局部EGFR,bFGF阳性细胞表达增强。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。