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The antitumor effects of sesquiterpene lactones scabertopin and isodexyelephantopin from the whole plant of Elephantopus scaber were studied in vitro on SMMC-7721,Hela and Caco-2 cancer cell lines.

采用四甲基偶氮唑盐比色法和琼脂糖凝胶电泳法等研究地胆草倍半萜内酯化合物scabertopin(1)和isodeoxyelephantopin(2)在体外对SMMC-7721、Hela和Caco-2三种肿瘤细胞增殖的影响。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果,选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验,采用快速银染法进行染色,随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索,鉴定了3个蛋白,它们是:1-氨基环丙烷-1羧酸合酶2,核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA,在含有甲醛的琼脂糖凝胶电泳后,转印到硝酸纤维膜上。

C. gloeosporioides mycelium grew well on media with fructose and glucose as carbon source while sorbose and starch promoted sporulation. The mycelia of C. dematium grew well with maltose as carbon source while starch promoted sporulation. Of the nitrogen sources tested, organic nitrogens were beneficial for both mycelial growth and sporulation but chemical nitrogens suppressed both growth and sporulation.

供试碳源中果糖和葡萄糖有利于胶孢炭疽菌的菌丝体生长,山梨糖和淀粉有利于其产孢;麦芽糖有利于黑线炭疽菌的菌丝体生长,淀粉有利于其产孢;氮源中有机氮有利于2种炭疽菌生长和产孢,化学氮源则表现抑制作用。

Establishment of a bovine mammary tissue culture system Bovine mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation.

1奶牛乳腺组织体外培养方法的建立采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织,通过LDH活力测定、台盼兰染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响,建立奶牛乳腺组织的培养体系。

In the tank farming test ,adding nanoparticle colloid of low concentration will increase the amount of soluble sugars and dissociative amino acid in the plants body ,thus it increased the anti-capacity of plants.But adding nanoparticle of high concentration can reduce the amount of soluble sugars and amino acid .

在水培条件下低浓度纳米胶液使玉米幼苗在生长过程中的可溶性糖和游离氨基酸的含量增加,增加了玉米幼苗的抗性。

Distinctive genomic fingerprints from DNA of Taraxacum mongolicum and its adulterants were generated with two long (20 24 mer )and one short (10 mer) random chosen primers Taraxacum mongolicum can be distinguished from six adulterants according to the banding p...

根据琼脂糖凝胶上显示的DNA带型差异可鉴别蒲公英和土公英混淆品。

Agarose gel electro- phoresis showed typical DNA ladders. These findings suggest that CP-induced apoptosis and/or cell death be involved in teratogenesis.

电镜观察所显示的细胞凋亡的形态学变化及DNA琼脂糖凝胶电泳的结果均提示CP致畸作用可能与其诱导的细胞凋亡有关。

When normal platelets are stimulated by thrombogen, ADP or collagen, the GPⅡb/Ⅲa receptor on the surface of platelet combine with fibrinogen that induces platelet aggregation.

正常血小板在各种刺激下,如凝血酶原,ADP,胶原诱导下被激活,其表面糖蛋白Ⅱb/Ⅲa受体由配体-非结合状态变为配体-结合状态,导致血小板聚集,形成血小板血栓。

Results Hypericin(75w tungsten lamp,10 min of light exposure,2μg/ml)had obvious cytotokic ef- feet on C6 cells, and the specific DNA...

结果金丝桃素在低剂量2μg/ml时,75W钨灯光照10min,即可对C6细胞株具有细胞毒作用;抽提其DNA做琼脂糖凝胶电泳可显示凋亡特有的DNA"阶梯"。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。