胶糖

Objective To study a new method for extracting agarose.

目的研究一种低成本制备琼胶糖的简便方法。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Results and Conclusion The extracting rate was 57%, gel strength reached 560 g/ cm~ 2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for bioche- mistry and molecular biology electrophoresis.

结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标，凝胶强度达到560g/cm2，提取率达57%，测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳。结论本方法提取的琼胶糖具有良好的电泳性能，适合用于生物化学和分子生物学的凝胶电泳研究。

Agarose ; basic resin ; gel strength ; gentian violet electrophoresis ; DNA gel electrophoresis

琼胶糖；强碱性树脂；凝胶强度；结晶紫电泳； DNA凝胶电泳

Scholaris and Coffea arabica (2.500 kg). Nine to eleven months after planting, A. scholaris trees grow very fast. Total biomass per plant of seed-propagated trees is 28.41kg, 3.21 times that of vegetatively propagated trees. Therefore, it is recommended that seed-propagated seedlings be used in A. scholaris production.

移栽定植后9~11个月是糖胶树生长的最快阶段；用种子进行苗木繁殖的糖胶树，其单株总生物量为28.41 kg，是营养繁殖的3.21倍，在生产上大面积推广时宜采用糖胶树种子进行幼苗繁殖。

Yummi Bears Fiber includes chicory root, an all-natural form of dietary fiber that sweeps away toxins and helps maintain your child's digestive health and regularity.

Yummi Bears纤维素胶糖包含菊苣茎，一种全天然的食物形式的纤维，能扫除体内毒素，保持儿童消化系统的健康和规则的良好状态。

EDWARD THE SEVENTH: Levitates over heaps of slain, in the garb and with the halo of Joking Jesus, a white jujube in his phosphorescent face

1349爱德华七世：（飘浮在成堆的被屠杀者尸体上面。他身穿滑稽的耶稣的衣裳，头上为耶稣的光晕所环绕。那张散发着磷光的脸上有一颗白色的枣味胶糖。

Unless you swapped its fangs for wine-gums or something that would make it less dangerous...

除非你把它的獠牙转换成酒胶糖什么的，使它变得不那么危险……

God. Save. Our. Sitting on his throne sucking red jujubes white.

上帝拯救我们的……坐在宝座上，把红色的枣味胶糖嘬到发白为止。

Locust bean and guar gums enhanced resistant starch content compared to gum arabic due to their glycosidic linkages and viscoelastic properties. The difference between locust bean and guar gums is ascribed to the low average length and frequency of unsubstituted mannose sequence in locust bean gum.

与阿拉伯胶相比，刺槐豆胶和瓜尔豆胶在增加抗性淀粉含量方面效果更显著，这主要归功于他们的糖苷键连接和粘弹性质瓜尔豆胶和刺槐豆胶的效果差异主要是源于刺槐豆胶中未取代的甘露糖链的平均长度以及频率更低一些。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。