胶粒

In order to investigate the formation, the optimal content of PCM and the latent heat release/absorption of EC microcapsules, the concentration of alginate, calcium chloride and ethyl cellulose were discussed.

制备混合粒时，以海藻胶浓度3%；5%氯化钙溶液经由孔径1.06mm的针头所制成的平均粒径为2.48±0.03mm，最佳包覆率为75.7%。

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段，对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验，对于质粒在硫杆菌中普遍存在的观点提出了质疑。

A loose, earthy, extremely fine-grained, natural sediment or soft rock composed primarily of clay-size or colloidal particles and characterized by high plasticity and by a considerable content of clay mineral and subordinate amounts of finely divided quartz, decomposed feldspar, carbonates, ferruginous matter, and other impurities; it forms a plastic, moldable mass when finely ground and mixed with water, retains its shape on drying, and becomes firm, rocklike and permanently hard on heating or firing.

中文解释=松散的、土质的、颗粒极细的天然沉淀物或软石，主要由黏土粒径大小或胶质的颗粒组成，特徵为具高可塑性，及大量的粘粒矿物，其次为的细小的石英、被分解的长石、碳酸盐、含铁物质和其他杂质；当精细研磨并和水混合后，可形成可塑的，适於模压的物质，乾燥后能维持形状，加热或烘烤时变得如岩石一般牢固，且长期保持坚硬。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜，含类似上皮层和固有层，但细胞层次不清；胶原膜上可见口腔黏膜上皮细胞黏附生长，并形成复层上皮样组织，但无成熟的桥粒及基底膜样结构；以Alloderm作为支架材料的构建物，出现正常上皮形态，含半桥粒及细胞指状突起等超微结构。

The sludge in SBMBR1 presented granular characteristics and settled very well. The sludge in SBMBR1was brown and the sludge flocs was smashed by the shear force due to high intensity of aeration. The sludge size decreased and the sludge flocs blended with water forming ropy solutions, and there was no distinguish interface between water and sludge. Low sludge age and high loading operation enabled the sludge in SBMBR1 keeping higher sludge activity and the MLVSS/MLSS ratio and SOUR values of SBMBR1 were higher than SBMBR2. Both in SBMBR1 and SBMBR2, there were large amount of zoogloea, bacillus, coryneform of bacteria, and pediococcus. However, in SBMBR1 there were also a lot of Protozoa like epistylis and etc. A certain amount of filamentous bacteria can also be found in SBMBR1. The biological species in SBMBR1 were much more abundant than that in SBMBR2.3. Membrane fouling on flat-sheets membrane was observed and analyzed with AFM, SEM and TEM. After the measurement of membrane surface EPS and membrane surface ETX analysis, the pollutants composition on the surface of flat-sheets membrane was calculated.

从外观观察SBMBR1反应器中的活性污泥呈浅黄色，随着运行时间的增加，污泥粒径逐步增加，SV、SVI值逐步下降，呈现出较强的颗粒特征，沉降性能良好；SBMBR2污泥呈土黄色，污泥絮体被高强度的曝气所产生的切应力所打碎，污泥粒径迅速降低，絮体较为细碎，和水相混合成均一粘稠的溶液，看不到泥水分界面；低泥龄、高负荷运行，使得SBMBR1可以保持较高的微生物活性，MLVSS/MLSS值、SOUR值均高于SBMBR2；两级反应器活性污泥中均有大量的菌胶团，长杆菌、短杆菌和球菌是构成主体，但SBMBR1活性污泥中含有大量固着型原生动物如累枝虫等，还存在着一定数量的丝状菌，生物相要比SBMBR2丰富。3、借助原子力显微镜、扫描电镜、透射电镜等手段对平板膜污染现象进行观测和分析，并通过膜表面污染物EPS测定、污染膜表面能谱分析，得出平板膜表面污染物的组成。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质粒，琼脂糖凝胶电泳检测质粒，用平板稀释法测定药物最低抑菌浓度，用试管内肉汤接合法和平板表面接合法进行接合转移试验。

The glutamate oxaloacetate transaminase isozyme of thirty-three accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species of the big-achene group and one species of the small-achene group were studied by means of polyacrylamide gel eletrophoresis.

用聚丙烯酰胺凝胶电泳技术时荞麦属8个种（含大粒组7个种和小粒组1个种）33份荞麦资源的谷草转氨酶同工酶进行了研究。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。