胶粒

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰，金颗粒呈团状、点灶状分布，特异性强，CTGF蛋白阳性标记主要位于粗面内质网，粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

The size of micelles is related to the chain architecture and the lengths of each block of the block copolymers.

胶束的粒径与共聚物分子链及各嵌段的长度有关。

The experiment results show that the recycled concrete with high fluidity, high durability or high strength can be produced by using low water-bind ratio, employing good quality super-plasticizer; adding of fly ash and colloidal silica and controlling the maximum size of recycled aggregate.

试验表明采取低水胶比、高效减水剂、粉煤灰和硅灰作为掺合料、控制再生骨料的最大粒径，可以制备出高流动性、高耐久性、高抗折强度的高性能路用再生混凝土。

Using single-seed gel-diffusion assay and electrophoresis method to measure the endo-β-mannanase activity in endosperm envelope of watermelon seeds during imbibition.

采用单粒种子凝胶扩散法和电泳法研究种子中β-半乳甘露聚糖酶的活性。

In this dissertation, based on glass microspheres and submicron silicon dioxide, the silver-coated core-shell shielding particles were prepared by liquid phase chemical deposition and layer by layer packing technology. The influences of the preparation conditions on the structure of the synthesized core-shell particles were also systemically studied. The conclusions are enumerated as follow:(1) Monodispersed submicron silicon dioxide spheres in the size range of 250～550 nm are synthesized by Sol-Gel method using ammonia as catalyst.

本文在前人研究的基础上设计出以微米级玻璃微珠为基核，采用液相化学沉积法和层层包裹法制备表面包银的核壳粒子电磁屏蔽剂，并对液相化学沉积法和层层包裹法的制备工艺进行了系统研究，取得了以下主要结论：(1)以氨水作催化剂，采用溶胶－凝胶法制备了粒径分布在250～550 nm之间的二氧化硅微球。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

For same felted and coated fertilizer, the shape of nutrient dissolved curves were similar in different sand column but dissolved ratios were not.

同一种胶结包膜型缓/控释肥料在不同粒径砂柱里氮磷钾溶出率曲线形状近似，但溶出率不同。

The morphology of microgel particle was obtained by using transmission electron microscopy in dry state.

在干燥条件下用电子显微镜对凝胶颗粒的形态进行了观察，发现其粒径分布相对较窄，基本保持球形。

Study shows that compaction and cementation can play a destructive role in Suining formation reservoir,while dissolution and fracturing can play a constructive role in that.The main facies of Suining formation are ferruginous-shale film,anhydrite,dolomite and calcite.The micro-fracture and grain-edged fracture mainly resulted from compaction fracturing,compaction slipping and tectonic dynamic force greatly improve the reservoir permeability.

研究表明：①对遂宁组储层起破坏作用的是压实作用、胶结作用和交代作用，而溶蚀作用、压裂作用则对储层起到建设性作用；②遂宁组最主要的成岩相组合为铁泥膜－硬石膏－白云石－方解石成岩相；③由压实破裂、压实滑动和构造动力形成的微裂缝、粒缘缝有效改善了储层的渗透性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。