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The change of R has also effects on hygroscopicity, thermodynamic stability, glass transition temperature and mechanical strength.

DMPA用量及R值的改变会影响乳液的外观、粘度、平均粒径、稳定性、固化速率等性能。R值的改变还会影响胶膜的吸水率、热稳定性、玻璃化温度、力学性能等性能。

The article studied Eisenberg model, Forsman model, Dreyfus model, Bonnoto Bonner, Longworth, Vaughan model and Core-shell model. A BAB-triblock copolymer was employed as macromolecular model. Minimized Gibbs free energy relates the micellar structure parameters to parameters of the triblock copolymer quantitatively. Furthermore, it could be explained theoretically that the micellar diameter and the equilibrium aggregation number both decrease with ion content.

然后讨论了BAB型三嵌段模型、Eisenberg模型、Forsman模型、Dreyfus模型、Bonnoto和Bonner、Longworth和Vaughan模型与核-壳模型,并以BAB型三嵌段离聚物为模型大分子,建立了胶束特征参数与聚合物结构参数之间的定量关系,从理论上解释了胶体粒径和聚集数随离子含量的增加而降低的原因。

AIM: To establish recombination plasmid pEGFP-NGB and to investigate the expression of pEGFP-NGB in culture neuroglia cells.

目的:构建重组小鼠质粒pEGFP-NGB并研究其在培养神经胶质细胞中的表达。

In this thesis, the hydrosol of CeO2 nanoparticles. the transparent organosol of CeO2 nanoparticles and CeO2 nanoparticles coated with DBS have been first prepared by colloidal chemical method, The CeO2 nanoparticles with various particle sizes were obtained by different calcination temperature.

本文首次采用胶溶法合成了CeO_2纳米水溶胶、透明的CeO_2纳米有机溶胶、表面包覆DBS的CeO_2纳米粒子,将该粒子在不同温度下进行热处理得到不同粒径的CeO_2纳米晶。

Part II: Characterization, package, and transfer of recombinant retroviral vector1 The pLXSN-GDNF construct, was characterized by DNA sequencing and compared to gene bank data to confirm expression of proper sequences.

第二部分 pLXSN-GDNF的鉴定、包装及转染1)对获赠的携带胶质细胞源性神经营养因子的逆转录病毒载体质粒进行测序鉴定。

The pALTER plasmid was successfully transfected into CHO cells and positive clones were screened and detected immunohistologically.

结果 含有人CD59两种突变体的重组pLATER质粒经酶切和琼脂糖凝胶电泳鉴定,得到长496 bp的电泳条带,与所插入片段完全相符。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The dissolubility of PPLA in water was (2.16~4.32)×10-2 mg·mL-1 according to eye judgment. The critical micelle concentration was 2.16×10-2 mg·mL-1 obtained from a fluorospectrophotometer. The self-assembled aggregates of PPLA in water were nanoscale spheres observed by a transmission electron microscope. The particle size and zeta potential tests by dynamic light scattering revealed that during preparation, the ratio of hydrophilicity/hydrophobicity, the aqueous medium and the pH had great influences; while dilution, freezing and pH change to basic condition had no effect after preparation, but pH change to acidic condition made the nanoparticles precipitate.

目测法得到其溶解度为(2.16~4.32)×10-2 mg·mL-1;荧光法得到聚合物的临界胶束浓度为1.12×10-3 mg·mL-1;透射电子显微镜观察显示该聚合物在水中的自组装聚集体为纳米级球形;动态激光光散射测试微球的粒径和Zeta电位发现,在微球的制备过程中,聚合物的亲/疏水性比例、水相介质及水溶液的pH值对它影响显著;而制备后,稀释和冷冻对它无显著影响,改变微球的环境pH值至酸性,出现聚集,至碱性无影响。

Pharmacodynamic action ; Xiaochaihu Oral Liquid ; Radix Bupleuri ; acute liver function damage of rat ; fever reaction of rabbit ; carbon particle clearance of mice ; delayed anaphylaxis reaction

昆明种小鼠;生理盐水;小剂量;碳粒廓清速率;小柴胡汤口服液;四氯化碳;鸡红细胞;迟发性过敏反应;角叉菜胶;药理作用

Through electron micrograph, sepiolite is found to have net fiber structure shaped by straw bundle.

海泡石一超细水泥的实验看出,超细水泥具有粒径小、比表面积大、水化速度快的特点,在加入海泡石后,海泡石并没有对超细水泥的水化速度产生很大的影响,能够使超细水泥的一些施工性能得到更好的改善,同样对超细水泥的胶结强度、抗折强度、抗冲击韧性起到提升的作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。