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Methods: 1. Experimental laparoscopic liver resection: We selected pigs as experimental models aiming to approach human beings physiological and anatomical condition. After CO2 pneumoperitoneum was established, the ligaments around liver were dissociated. Livers were cut off by the methods such as clamp dissection, microwave tissue coagulator, ultrasound dissector, Ligsure dissection, hand-assisted, short abdominal incision laparoscopy-assisted, and Endo-GIA switcher. The operation time and bleeding volume were compared between different methods of laparoscopic liver resection. The advantages and limitations of different methods were summarized. 2. Clinical laparoscopic liver resection: 15 cases with tumors in segment V of anterior right lobe or segments II, III of left lateral lobe or segment IV of left internal lobe were selected in this study. The liver diseases included hepatocellular cancer, hepatic cavernous hemangioma and hepatic focal nodular hyperplasia. The ligaments around liver were dissociated after CO2 pneumoperitoneum established. Laparoscopic liver resection were carried out by some of the methods combined such as clamp dissection, microwave tissue coagulator, hand-assisted, short abdominal incision laparoscopy-assisted, ultrasound dissector, rotation and suction dissector.

1、实验性腹腔镜肝切除:以猪为实验动物,旨在接近人体的生理、解剖环境下进行实验,建立操作空间后游离肝脏周围韧带,采用钳夹分离切肝法、微波刀切肝法、超声刀切肝法、Ligsure切肝法、手辅助腹腔镜肝切除法、小切口腹腔镜辅助切肝法、Endo-GIA切肝法等方法实施腹腔镜下肝切除,比较不同切肝方法的手术时间、出血量,总结各种方法的优越性及其局限性。2、临床腹腔镜肝切除:选择肿瘤位于肝右前叶第Ⅴ段、肝左外叶第Ⅱ、Ⅲ段及左内叶第Ⅳ段的15例患者,病种包括原发性肝癌、肝海绵状血管瘤、肝脏局灶性增生,建立气腹后游离肝脏周围韧带,采用钳夹分离断肝、微波刀固化后断肝、手辅助腹腔镜肝切除、小切口腹腔镜辅助肝切除、超声刀断肝、旋吸断肝等多种方法分离切线肝组织,暴露管道结构,再采用高频电凝、钛夹夹闭、缝扎止血、止血纱布覆盖、氩气刀止血、生物胶止血、Endo-GIA处理等多种方法相结合处理肝断面,统计手术完成情况、手术时间、手术出血量、术后并发症及术后住院时间等,评价腹腔镜肝切除的可行性及安全性。

A schematic model was proposed to illustrate the occurrences of various lithologies and lithofacies associations. The erosional surfaces on siliciclastic mudstones, funnel-shaped structure, and the exhuming of massive cold-seep carbonates exposed might have been occurred concurrently during a tectonic unstable time in SW Taiwan. The deposition of fossiliferous mudstone interfingered with the conglomerate lithofacies, and represented the rapid facies transition from siliciclastic (non-carbonate) into carbonate environment.

综合岩相观察结果,建构一个符合台湾西南部更新世珊瑚礁与基底泥岩接触关系的沉积相模式;其中,基底泥岩顶部的不规则冲蚀面、充填碳酸盐胶结泥岩砾与碳酸岩烟柱的漏斗状沉积,以及块状冷泉碳酸盐岩的出露,可能均为区域地质构造活动时期所造成;至於与含化石泥岩同时沉积的砾岩相,可能均为基底泥岩在快速浅化的过程中所沉积,标志古环境由矽质碎屑快速转变为碳酸盐环境的过程。

Pathologically, it showed palisaded granulomas containing degenerated collagen, predominantly lymphohistiocytic infiltration with scattered eosinophils leading to dermal fibrosis.

在病理下可发现有栅栏状排列的组织球围绕著变性的胶原纤维,而浸润的发炎细胞主要由淋巴球、组织球与散在性的嗜伊红球至后续的真皮层纤维化。

A glass die, a micro replication characteristic of PDMS and an electroplating technology are used to make the three-dimensional microelectrode array. A precise cutting machine is used to cut the glass to form a column array and a mixture of HF and NH4F is used to produce the corresponding structure serving as the die of the micro replication of PDMS. The photoetching technology is left out and the limitation of a photoresist on the dimension of the three-dimensional structure is eliminated; therefore the longer microelectrode array can be made. The length of the microelectrode array depends on the incision depth of the glass and the electroplating technology of deep hole of the metal.

本发明利用玻璃模具、PDMS的微复制特性和电镀技术制作三维微电极阵列,用精密切割机切割玻璃形成柱状阵列,用HF和NH 4 F的混合液腐蚀出相应的结构作为PDMS微复制的模具,免去了光刻工艺,消除了光刻胶对三维结构尺寸的限制,可以制作更大长度的微电极阵列,这一长度决定于玻璃的切割深度和金属的深孔电镀技术。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。

The sludge in SBMBR1 presented granular characteristics and settled very well. The sludge in SBMBR1was brown and the sludge flocs was smashed by the shear force due to high intensity of aeration. The sludge size decreased and the sludge flocs blended with water forming ropy solutions, and there was no distinguish interface between water and sludge. Low sludge age and high loading operation enabled the sludge in SBMBR1 keeping higher sludge activity and the MLVSS/MLSS ratio and SOUR values of SBMBR1 were higher than SBMBR2. Both in SBMBR1 and SBMBR2, there were large amount of zoogloea, bacillus, coryneform of bacteria, and pediococcus. However, in SBMBR1 there were also a lot of Protozoa like epistylis and etc. A certain amount of filamentous bacteria can also be found in SBMBR1. The biological species in SBMBR1 were much more abundant than that in SBMBR2.3. Membrane fouling on flat-sheets membrane was observed and analyzed with AFM, SEM and TEM. After the measurement of membrane surface EPS and membrane surface ETX analysis, the pollutants composition on the surface of flat-sheets membrane was calculated.

从外观观察SBMBR1反应器中的活性污泥呈浅黄色,随着运行时间的增加,污泥粒径逐步增加,SV、SVI值逐步下降,呈现出较强的颗粒特征,沉降性能良好;SBMBR2污泥呈土黄色,污泥絮体被高强度的曝气所产生的切应力所打碎,污泥粒径迅速降低,絮体较为细碎,和水相混合成均一粘稠的溶液,看不到泥水分界面;低泥龄、高负荷运行,使得SBMBR1可以保持较高的微生物活性,MLVSS/MLSS值、SOUR值均高于SBMBR2;两级反应器活性污泥中均有大量的菌胶团,长杆菌、短杆菌和球菌是构成主体,但SBMBR1活性污泥中含有大量固着型原生动物如累枝虫等,还存在着一定数量的丝状菌,生物相要比SBMBR2丰富。3、借助原子力显微镜、扫描电镜、透射电镜等手段对平板膜污染现象进行观测和分析,并通过膜表面污染物EPS测定、污染膜表面能谱分析,得出平板膜表面污染物的组成。

Using 11C--PK11195 positron emission tomography, we investigated microglial activation in HD presymptomatic gene carriers, its relationship with striatal neuronal dysfunction measured with 11C-raclopride PET, and the role of PK PET as a possible marker of subclinical disease progression in PGCs. Eleven HD PGCs underwent PK and RAC PET. Their results were compared with those of healthy controls.

利用11C--PK11195电子发射断层扫描,我们研究了HD的基因携带者症状发生前小神经胶质细胞的活化情况,它与纹状体的神经元的机能障碍的关系,以及PET可能作为PGCs亚临床症状进展的指标。11位HDPGCs接受了PK和RACPET检查,其结果与健康对照人群进行比较。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Therefore, the resonant cavity that is composed of porous structures produced by wind erosion, water erosion, chemical dissolution and gel of SiO2 is vital to sound-producing of booming dune.

因此自然沙粒表面由风蚀、水蚀、化学溶蚀及硅凝胶沉淀等多种因素所形成的多孔状结构所构成的共鸣腔,是鸣沙发声的关键所在。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能,并可抑制Hela细胞对裸鼠的致瘤功能(P.01);维生素A的抑瘤功能强于4HPR(P.01);电镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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