胶原质

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Fig 3Lots of rough endoplasmic reticula, mitochondrid an d collagen in the cytoplasm of fibroblast in control group

图3对照组的瘢痕成纤维细胞粗面内质网、线粒体丰富，形态结构正常，周围胶原纤维较多TEM×5200

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象，线粒体少，胞核畸形，胞内有吞噬颗粒，细胞周围胶原纤维较少TEM×5200

Therefore, collagen metabolism is the center of the extracellular matrix remodeling in intracranial saccular aneurysms, while the essence of the remodeling process is a kind of autoimmunological and inflammatory reaction.

因此，胶原代谢是囊性动脉瘤细胞外基质重塑的核心内容，而重塑过程的实质是一种以细胞免疫为主的免疫炎性反应。

Low reaction of fetus to inflammation, different extracellular matrix, component of collagen fiber and cell factors may take part in scarless healing process. Regulation of gene expression may play an important role in the process. But the concrete mechanism of scarless healing is not clear yet and still needs more researches and elucidations.

胎儿对炎症的低反应性、胎儿不同的细胞外基质、胶原纤维的成分、细胞因子可能也参与了无瘢痕愈合过程、基因表达调控在其过程中可能也起重要作用，但无瘢痕愈合的具体机制还不明了，有待进一步研究阐明。

Objective: To investigate the reconstruction of type Ⅰ and Ⅲ interstitial collagen of sinuatrial node and the intervention role of insulin in type Ⅱ diabetes mellitus rat.

目的：探讨Ⅱ型糖尿病大鼠窦房结内Ⅰ、Ⅲ型间质胶原重构及胰岛素对其的影响。

P＜0.01. 28d, q=4. 0452, p＜0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示（UV-B 14天）：病毒对照组角膜基质层高度水肿、增厚，并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生，胶原纤维肿胀、分解等，说明除HSV-1对角膜组织直接破坏外，炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

After observing the specimens from JMA-m groups, we have found that epithelium and endothelium of cornea full, the lesion are light edema at stromatic layer. One small amount of inflammatory-cell infiltrate presents the collagen fiber lack of rules at shallow stromatic layer.

观察角膜安的结果表明，中药中剂量组角膜上皮和内皮层完整，基质轻度水肿，浅基质层内少量炎性细胞浸润，胶原纤维走行欠规则，整体改变较低剂量组减轻。

The bone mineral density of the subchondral cancellous bone as well as levels of urinary N-telopeptide of type I collagen and serum osteocalcin were measured.

我们检测了软骨下松质骨的骨矿物质密度以及尿Ⅰ型胶原N末端肽和血清骨钙素的水平。

The ultimate result of chronic HC injury is the accumulation of extracellular matrix with high density type I collagen within the subendothelial space of Disse, culminating in cirrhosis with hepatocellular dysfunction.

慢性HC损伤的最后的结果是在内皮下间隙高密度的1型胶原的细胞外基质堆积，最后为肝细胞的功能障碍、肝硬化。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。