胶原质

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Acellular allogeneic dermal matrix is the dermal replacement of heterogenous human skin in which the cellular elements have been removed by special technique and basement membrane complex have been remained. It becomes a kind of 3D porous acellular dermal scaffold. It supports fibroblast infiltration, neovascularization and epithelialization in the absence of an inflammatory response.

异体脱细胞真皮基质（acellular allogeneic dermal matrix， ADM）来源于正常的异体皮肤组织，经过特殊的理化处理去除真皮中的细胞成分，保留原有的胶原三维结构和完整的基底膜，成为一种多孔的三维膜性支架，其空间网状结构有利于细胞的增殖、毛细血管增生，加速组织的愈合过程，移植后可以迅速被血管化和自体细胞重建。

The D values of theophylline and benzocaine in GTA-crosslinked and epoxy-crosslinked films were about one third of the D values in the untreated films.

经过GTA或是epoxy交联的胶原蛋白薄膜，两种药物在其中的外显质传系数都降低为原值的三分之一左右。

Tissue engineering Cartilage ; Collagen-hyaluronan ; Cartilage cell ; Rabbit

组织工程软骨；胶原-透明质酸；软骨细胞；兔

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

Results:Periodontal ligament cells formed confluent layer on Ti after 7 days .

结果 ：牙周韧带细胞在纯钛表面生长良好，14天后形成单层，形成大量细胞基质，其成份主要为Ⅰ型胶原。

In the pathological proceeding of pulmonary fibrosis, myofibroblast plays an important role, such as: the abnormal extracellular matrix deposition causing by the remarkable capability of collagen synthesis, derogation of the lung compliance owning to the contractibility of myofibroblast,secreting many cytokines,which contribute to the damage of the alveolar epithelial cells.

肌成纤维细胞在PF的病理进程中扮演着重要的角色，其胶原合成能力强可造成细胞外基质的异常沉积、具收缩性使肺顺应性下降、分泌多种炎性介质加重肺泡上皮损伤。

In corium, collagen and elastin forms the 3D structure and are filled with hyaluronic acid, thus creating tender and elastic sin.

在真皮层中，胶原蛋白与弹性蛋白形成立体结构，保水成分透明质酸填充其间，为肌肤带来水嫩与弹力。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。