胶原质

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

BACKGROUND: Based on the previous study, the complex of alginate gel and bone marrow mesenchymal stem cells can induce chondrogenesis in vivo and secrete type Ⅱ collagen.

背景：课题组前期已经发现藻酸盐凝胶/骨髓间充质干细胞复合物能在体内形成软骨，并能稳定分泌Ⅱ型胶原。

Abstract］ Objective To study the influence of different residual cornea bed of laser in situ keratomileusis on the collagenic construction of cornea through investigating the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in cornea cells for different residual stroma bed after LASIK for six months.

目的 研究不同切削厚度的准分子激光原位角膜磨镶术（laser in situ keratomileusis，LASIK）后兔眼基质金属蛋白酶（matrix metalloproteinases，MMPs）及基质金属蛋白酶组织抑制剂(tissue inhibitors of metalloproteinases，TIMPs)的表达，以探讨LASIK手术的不同切削深度对角膜的胶原结构的影响。

Ingredients: Arbutin 2%, Electrodialyzed Deep Sea Water, Mink Oil, Emu Oil, Borage Seed Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Centella asiatica L.

主要成分：含熊果苷2%，海洋深层水，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，玫瑰果油，突厥蔷薇，蜂胶，积雪草，黄芩，甘草，金银花瓢虫，银杏叶，人参，洋甘菊，葛根等提取物

Ingredients: Electrodialyzed Deep Sea Water, Disodium Laureth sulfosuccinate, Coco-Betaine, Mink Oil, Emu Oil, Jojoba Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Rose Hip Oil, Borage Seed Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Morus alba L.

主要成分：含海洋深层水， Disodium Laureth sulfosuccinate，可可甜菜碱，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，玫瑰果油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，弹性蛋白水解，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，突厥蔷薇，蜂胶，薄荷，芝麻，何首乌，银杏叶，枸杞，荨麻腿子属，洋甘菊，葛根等提取物

CD34 hematopoietic stem/progenitor cells reside in the bone marrow in close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal cells, and various extracellular matrix molecules. We used a bioartificial matrix of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex vivo expansion of HSCs.

CD34 的造血干细胞/祖细胞存在于接近骨内膜表面的骨髓中，由成骨细胞、间充质细胞以及各种细胞外基质分子所包绕，我们采用生物人工合成的纤维胶原I

CD34+ hematopoietic stem/progenitor cells reside in the bone marrow in close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal cells, and arious extracellular matrix molecules. We used a bioartificial matrix of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex io expansion of HSCs.

CD34+的造血干细胞/祖细胞存在于接近骨内膜表面的骨髓中，由成骨细胞、间充质细胞以及各种细胞外基质分子所包绕，我们采用生物人工合成的纤维胶原I

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

It starts transmitting information by integrating with lagen and stromatin by irritating osteocytes and chondrocytes to form rapidly extracellular matrix so as to promote repair of bone and cartilage injury.

转化生长因子β通过刺激骨、软骨细胞合成胶原和基质蛋白，快速形成细胞外基质，达到其促进骨、软骨损伤修复的作用。

Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果］韧带支架材料的柔韧性强，拉力测试的负荷－拉伸曲线与韧带的拉伸曲线相似，其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa；体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征，能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质；支架材料无细胞毒性，人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。