胶原质

Objective To study the relationship between GFAP and the malignant degree of astrocytoma.

目的 研究胶原纤维酸性蛋白与星形胶质细胞瘤恶性程度的关系。

Dimethicone ,Cyclopentasiloxane,Dimethicone/Vinyl Dimethicone Crosspolymer,Polysilicone-11,Glycoproteins,Panax Ginseng Root Extract,Equisetum Arvense Extract,Silica Dimethyl Silylate ,Bisabolol,Tocopheryl Acetate ,Sodium Hyaluronate,Ursolic Acid,Atelocollagen,Sodium Chondroitin Sulfate,Ethylhexyl Methoxycinnamate,Polyglyceryl-2 Tetraisostearate ,Glycerin ,Water,Alcohol,Butylene Glycol ,Lecithin,Xanthan Gum ,Mica,Fragrance ,Benzyl Alcohol,Linalool ,Hydroxycitronellal,Alpha-Isomethyl Ionone ,Amyl Cinnamal,Hexyl Cinnamal,Palm Tree Extract,Geraniol,Benzyl Benzoate,Butylphenyl Methylpropional,Phenoxyethanol,Methylparaben,Isopropylparaben,Butylparaben ,Isobutyl Paraben,Titanium Dioxide,Carmine

地美司康，环戊硅氧烷，聚二甲基硅氧烷/乙烯基聚二甲硅氧烷交联聚合物，聚硅氧烷-11，糖蛋白，人参根萃取，问荆萃取，二甲基甲硅烷基化硅石，没药醇，醋酸盐维他命E，透明质酸钠，熊果酸，去端肽胶原，硫酸盐软骨素，4-甲氧基肉桂酸-2-乙基己基酯，聚甘油-2 四异硬脂酸酯，甘油，水，酒精，丁二醇，卵磷脂，山羊胶，云母，香料，苯甲醇，沉香醇，羟基香茅醛，α异甲基紫罗酮，戊基肉桂醛，己基肉桂醛，棕榈树萃取，香叶醇，苯甲酸苯甲酯，丁苯基甲基丙醛，苯氧乙醇，羟苯甲酯，羟苯丙酯，羟苯丁酯，二氧化钛，胭脂虫红

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Primary Ingredients: Mint cooling factors, herbage essence, collagen, hyaloplasm acid, Lubrajel, elastic silicon oil, aloe gel juice and etc..

主要成份：薄荷清爽因子、草本精华、胶原蛋白、透明质酸、芦芭胶、弹性硅油、芦荟凝胶汁等。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法，0.1%胶原酶和0.1%透明质酸酶消化20 min，0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min，就可以使睾丸生殖细胞充分分离处出来，每克睾丸组织克获得8.4±2.6×106个细胞，其中PGP9.5 精原细胞占(19.7±5.3)%，c-kit 精原细胞占总数的(23.8±3.6)%。

Demonstrative green tea can reduce gland purine to be damaged to the qualitative pathology between bandicoot kidney, green tea has fight the action that qualitative fiber changes between kidney, and can restrain hyperplasia of fibrous of I,Ⅲ collagen, this may be green tea qualitative fiber is changed between kidney of prevention and cure, one of mechanisms that defer chronic kidney failure to develop a process.

说明绿茶能够减轻腺嘌呤对大鼠肾间质的病理损害，绿茶有抗肾间质纤维化的功能，并且能抑制I、Ⅲ型胶原纤维的增生，这可能是绿茶防治肾间质纤维化、延缓慢性肾衰竭发展进程的机制之一。

RESULTS AND CONCLUSION: The seeded cells had chondrogenesis in scaffolds under certain conditions.

结果与结论：种子细胞在三维支架中特定条件下有成软骨效应，骨髓间充质干细胞在含20%透明质酸的胶原/透明质酸支架扩增少于3代有成软骨效应，软骨细胞传代数更高仍然有成软骨效应，但脂肪源性成熟基质细胞成软骨效应能力较弱。

Results Microscopically, different degrees of hepatocytic degenerative changes and hyperplasic fibrous tissue and typical false lobule formation could be seen. Timm's staining result showed uneven distributed black granular deposits in the hepatocytes. No specific PAS staining was observed. Utrastructurally, the mitochondria were increased in volume and dramatically different in shape. The number of lysomes were increased.

结果 光镜下肝细胞表现为不同程度的退行性变，胶原纤维增生以及典型假小叶形成；Timm's染色阳性，发现不均匀分布黑色颗粒或团块样物质沉积；PAS染色则普遍缺乏特异性染色；超微结构显示线粒体形态多样，体积增大，溶酶体增多，粗面内质网管腔扩张，附着核糖体的脱颗粒，以及胞质水肿，质膜溶解。

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰，金颗粒呈团状、点灶状分布，特异性强，CTGF蛋白阳性标记主要位于粗面内质网，粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。