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胶原

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The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中,加入用DMEM配制的0.15%胶原酶II溶液,水浴箱中作用1.5h,至组织呈絮状,用0.02%的EDTA清洗,然后,加入0.125%胰蛋白酶溶液,于37℃水浴振荡消化,即可得到分散的平滑肌细胞,用10%新生牛血清的培养液终止酶的作用。加入含20%新生牛血清的培养液,调节细胞密度为5×104~5×105个/L,接种,放入5%CO2、37℃培养箱中培养。当细胞达到70%~80%融合时即可按常规方法传代。

Department of Pulmonary Disease, Qilu Hospital of Shandong University, Jinan 250012, ChinaObjectiveTo study the relationship between transcription factor T-bet/GATA -3 and expression of α-smooth muscle actin and type Ⅰ collagen in asthma.

目的观察大鼠肺转录因子T细胞表达的T盒/GATA连接蛋白-3 (GATA3)对肺支气管α-平滑肌动蛋白和Ⅰ型胶原的调解作用,探讨其比率与哮喘早期气道重塑的关系。

1Department of Cardiovasology, Tangdu Hospital, Fourth Military Medical University, Xi an 710038,China, 2Institue of Geratic Cardiology, PLA General Hospital, Beijing 100853, China

目的:研究衰老大鼠肺部感染时血浆血管加压素(Arginine vasopressin, AVP)浓度的变化及其与心脏胶原蛋白之间的关系。

The cartilage of germinative layer and basal layer grew better than that in bfgf group, magnetic group.

结果表明:磁场结合bfgf组在4周时基底层的软骨样组织增生,而磁场组、bfgf组少量软骨样组织增生。8、12周结合组透明样软骨层明显增厚,免疫组化ⅱ型胶原多。

Objective To explore the value of detecting urinary type Ⅳ collagen in diagnosis of glomerular diseases.

目的 探讨尿中Ⅳ型胶原在肾小球疾病诊断中的应用价值。

Results The pathological symptom of DN occurred in all model rats.The renal pathological structure,thickening of glomerulose basement membrane and increasing of fibronectin and collagen IV expression in renal tissue were significantly de-creased in HDC by given HDC.

结果 高血糖组大鼠均出现DN病理学特征,黄丹益肾胶囊用药组大鼠DN病理学改变明显减轻;肾小球基底膜增厚较模型组对照组小;肾组织纤维连接蛋白、肾组织Ⅳ型胶原表达明显低于模型对照组。

Objective To explore the histological fate of human collagen and autologous fat injected into vocal cords in treating glottic insufficiency.

目的 探讨国产人胶原狗声带注射的组织学转归及其在矫治声门闭合不全中的应用价值和前景。

Hydroxyprolisilane CN, mineral spring essence,β-glucosan, hyaluronic acid, collagen.

羟基脯氨酯硅烷醇 CN ;矿泉精华;β一葡聚糖;透明质酸;胶原蛋白

The results show that when BSA was added with Gluma, polymerization of HEMA occurred.

分别用戊二醛、HEMA和Gluma与羟基磷灰石、I型牛胶原蛋白、牙本质和模拟牙本质液作用。

AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampusderived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering.

目的:评价豚鼠海马神经干细胞与2种不同载体材料(胶原蛋白海绵和明胶海绵)的生物相容性,探讨它们作为周围神经组织工程载体材料的可行性。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?