胶原

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞，P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%，20%，50%，80%，100%)的胶原/透明质酸支架中，在无血清培养液中三维培养。2周后，用SO染色和免疫组织化学染色分析，观察硫酸软骨素和Ⅱ型胶原合成情况。

Collagen is albumen of structure of a kind of fibroid, say for collagen albumen.

胶原是一种纤维状的结构蛋白，称之为胶原蛋白。

Water, Glycerin, Methylsilanol Mannuronate, Sodium Carboxymethyl Beta-Glucan, Sodium Hyaluronate, Palmitoyl Oligopeptide, Palmitoyl Tetrapeptide-7, Madecassoside, EGF *, FGF *, Telomerase *, Panthenol, Borago Officinalis Seed Oil, Anthemis Nobilis Flower Extract, Tocopherol, Tocopheryl Acetate, Cetyl Palmitate, Citrus Aurantium Dulcis Peel Oil, Citral, Citronellol, Limonene, Linalool, Zea Mays Starch, Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Hydrolyzed Corn Starch, Hydrolyzed Corn Starch Octenylsuccinate, PEG-33, PEG-8 Dimethicone, Hydroxyethylcellulose, PEG-14, Xanthan Gum, Carbomer, Polysorbate 20, Caprylyl Glycol, Phenoxyethanol, Methylparaben.

主要有效成分是：聚羧基甲基β葡萄糖的钠盐，保湿锁水；透明质酸，高效保湿；寡胜肽；四胜肽；羟基积雪草甙，抗炎症，促进胶原蛋白的合成；表皮生长因子EGF，促进细胞新陈代谢；成纤维细胞生长因子FGF，促进胶原蛋白合成；端立酶，一种RNA与蛋白的复合体，可通过合成新的端粒而修补受损的DNA端粒，使细胞持续不停的增生，也就是利用成人干细胞转变为全新的细胞。维他命B5；琉璃苣籽油，含维他命和矿物质；洋柑橘萃取液，舒缓，抗过敏；维生素E；醋酸盐维生素E；橙皮油；水解玉米淀粉。

Chitosan-Collagen-GAGs collagen gelart ificial skin is a new collagen gel living artificial skin which has certain antibacterial ability and stratified epiderm is and dense dermis structure like normal skin.

壳多糖-胶原-GAGs胶原凝胶人工皮肤是一种新型、有一定抗感染能力的活人工皮肤。

Ones this guar gum is a kind of purely natural vegetable gule,has stronger gluey function.Hydroxyl third base guar gum is got through modifying in chenistry by the guar gum powder,has a lot of excellent characteristics,water insoluble matter and residue is also less than the former pomer,applied in a extensive ronge.

瓜尔胶是一种纯天然植物胶，具有较强的增粘作用，羟丙基瓜尔胶是有瓜尔胶粉经化学改性后得到的，忽悠许多优异特性，水不溶物及残渣均较瓜尔胶原粉有较大幅度的降低，应用范围广泛。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

Three kinds of molecular weight determination methods, i.e., size exclusition chromatography, sodium dodecyl sulphate-polyacrylamide gel electro phoresis and high performance liquid chromatography / electro spraying ionization-mass spectrometry (HPLC/ESI-MS) are introduced to study of the attribute of collagen hydrolysates.

应用体积排阻色谱、聚丙烯酰胺凝胶电泳、高效液相与电喷雾质谱联用(HPLC/ESI-MS)这三种蛋白质分子量分析测试技术，来表征胶原水解产物的分子量，以寻求较好的胶原水解产物分子量的测试方法

Results ET-1 increased the synthesis of soluble type I collagen in different periods besides the 24h group, and had no effects on the type III collagen; immunohistochemical staining revealed that ET-1 also increased the synthesis of unsoluble type I collagen.

结果 ET-1较对照组除24小时时相外，均有不同程度的促HDPC可溶性I型胶原合成作用，但对III型胶原作用不明显；经免疫组化染色证实：ET-1尚可同时促进细胞非溶解性I型胶原合成。

When cultured in three-dimensional matrix, the cells were elongated and migrated into the gel, with a phenotype similar to that in the process of angiogenesis and vasculogenesis in vivo .

在二维平面培养中，原代心脏血管内皮细胞呈铺路石样排列；而在三维胶原培养模式中，内皮细胞呈长梭状形态，并迁入胶原培养介质中，和体内血管新生及血管生成过程中的内皮细胞活化表型相似。

Results The maximum absorbance of the purified collagen appeared at 230nm, with correspondence to type Ⅰ collagen, two close bands on SDS-PAGE (mol. wt 98 000) suggested the purified product was type Ⅰ collagen.

结果：提取的胶原紫外分光光谱最高吸收峰值为230nm左右；聚丙酰胺凝胶电泳呈现2条相邻带（相对分子质量98000左右），符合Ⅰ型胶原的特性。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。