胶原

To some degree, this study revealed the relationship between ossein and bone miner and the important preventive and therapeutic effects of ossein on OP. It provides theoretical and experimental basis for the research and development of effective prescriptions to cure OP. Limited by the actual experimental situation, we only did some study on the synthesis and content of type Ⅰ collagen. Further study on the changes of decomposition and structure of type Ⅰ collagen are needed to be done.

本课题的研究，在一定程度上揭示了骨胶原纤维和矿物质之间互根互用的辨证关系，及Ⅰ型胶原在骨质疏松症的发病和治疗中所起的重要作用，并为开发研制治疗本病的有效中药复方提供了理论和实验依据，但由于条件所限，本研究仅就Ⅰ型胶原的合成及其含量变化做了初步观察，而本病中骨胶原纤维的分解代谢及其结构的改变，尚待进一步探讨。

Results: The ratio of I and Ⅲ collagen fibers in the sinoatrial nod increased with aging. Type I was the majority while type Ⅲ decreased during aging.

结果：窦房结胶原纤维随年龄的增高有增高的趋势，发现胶原纤维以1型为主，3型胶原纤维随年龄的增高有减少的趋势。

Reconstruct ion of interstitial collagen in sinuatrial node occur since the rat diabetes has been duplicated for 16 weeks. The unbalance between collagen type Ⅰ and type Ⅲ in sinuatrial node of diabetes rat can be reversed by insulin treatment.

Ⅱ型糖尿病大鼠窦房结16周后出现间质胶原重构，胶原类型比例失衡；Ⅱ型糖尿病大鼠行胰岛素降低血糖后可不同程度下调窦房结间质的沉积，一定程度上逆转胶原类型比例紊乱。

They were composed of wavy and tendinous collagen fiber fascicles, among which a small amount of loose tissue was found to be intermingled with vessels.

桡舟韧带、桡舟头韧带和桡月韧带的组织结构相似，由肌腱样的胶原纤维束组成，胶原纤维束之间有少量的疏松组织，其间有血管分布，胶原纤维束呈小的波浪状。

Results There was moderate or strong negative correlation among the indexes of the degeneration of intervertebral disc including the relative area of vascular bud, the ratio of the uncalcified and the calcified layer, the quantitation of collagen type Ⅱand the indexes of osteoporosis including the thickness ratio of cortical bone, the relative area of bone trabecula, the density of femoral bone and vertebral body bone, the max stress and straining of bone.

结果 骨质疏松相关指标骨皮质厚度指数、骨小梁相对面积、股骨密度、椎体骨密度与椎间盘退变指标终板血管芽相对面积、非钙化/钙化层比值、Ⅱ型胶原定量之间存在中度到强度的负相关，而与Ⅹ型胶原表达存在中度到强度的正相关，其中椎体骨密度与软骨终板的Ⅹ型胶原相关性最强。

The RF electricity of ice adopts radio frequency technique of the RF dual pole. The frequency can make the water element of the cell which at the certain depth shake at a high speed, it can produce the quantity of heat at 45-60℃ through the quick circumvolved friction. Because the collagen fibre is shrinked, the wrinkle can be tighted. Then the light pyrolysis stimulates collagen and elasticity fibre to become more and arrange once again so as to reach the function of dispelling wrinkle and shrinking pore.

冰电除皱系统采用RF双极射频技术，发射的频率可以在皮下的特定深度内使细胞内的水分子产生高速震动，通过这种高速的旋转摩擦从而产生热量45-60℃，胶原纤维收缩，即刻松弛的皮肤、皱纹被拉紧，随后，真皮层中产生的热效应使胶原增生、重新排列、数量增加，同时修复老化受损的胶原，达到即刻紧肤、长效除皱抗衰老的效果。

PART 1 EXPRESSION OF MMP/TIMP IN SKELETAL MUSCLE OF ARTERIAL OCCLUSION DISEASE MODELS OF DOG'S HIND LIMB Objective To study the distribution changes of typeⅠand typeⅢcollagen in skeletal muscle of arterial occlusion disease models of dog's hind limb and the expression of MMP-2/TIMP-2 in skeletal muscle of arterial occlusion disease models of dog's hind limb. To investigate the significances of collagen metabolism and MMPs in skeletal muscle remodeling due to ischemia.

第一部分MMP/TIMP在犬后肢动脉闭塞性病变模型骨骼肌中表达的变化目的研究Ⅰ型胶原、Ⅲ型胶原在犬后肢动脉闭塞性病变模型骨骼肌的分布变化以及MMP-2、TIMP-2在犬后肢动脉闭塞性病变模型骨骼肌表达的变化，探讨胶原代谢和基质金属酶系统在缺血导致的骨骼肌重塑中的意义和作用。

Results T-2 toxin could cause rat articular chondroncytes degeneration, necrosis, and appeared many blank areas free cells. Weigert/Van Gieson staining showed obvious collagen fasciculi emergence could be seen. Scanning electronic microscope showed the surface of articular cartilage was wave and rough. Collagen fasciculi ruptured and stack up, and presented a typical 揳rticular dryness?phenomenon. ELISA results indicated, at the stage of 3 month, the level of rat serum CTX-II in T-2 toxin group was higher than that in control group, and the difference had a markedly significance.

结果 T－2毒素可致大鼠关节软骨细胞变性、坏死，出现大面积无细胞区，胶原染色可见明显的&胶原显现&；扫描电镜显示，关节面起伏不平，表面粗糙，胶原纤维断裂、剥脱，关节表面布满裂片状凸起，呈典型的关节&干燥&现象；ELISA检测结果表明， T-2毒素组大鼠血清CTX-II水平在3个月时较对照组明显增加，差异具有统计学意义。

Results It was shown that proliferation of fibroblasts and collagen synthesis were stimulated by wound fluid remarkably on 5 to 8 days after wounding, and that 6 Gy to total-body irradiation wound decrease this effect.

结果 在伤后5～8天，伤口液刺激FBS增殖和胶原合成的作用最强，6 Gy全身辐射后，伤口液刺激FBS增殖和胶原合成的作用都明显降低，而苯妥英钠对非辐射组和辐射组伤口液刺激FBS增殖和胶原合成的作用都有明显的提高。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。