胶原

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞，对原代至第5代rgcs进行细胞生长动力学分析，阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖，α sma和ⅱ型胶原的表达。

Substantial collagenous fibril proliferation at the inner side and around the endoneurium was also observed.

电镜下，雪旺细胞出现在神经内膜管内，神经内膜管下及内膜管周围有大量胶原原纤维增生。

The products that we have been developed are: intermediate include Protoporphyrin,, protein peptone, plant peptone; Flavor material include hydrolyzed vegetable protein, meat flavor protein powder; food material include compound amino acid powder, soybean peptide,soybean polypeptide,blood peptide, collagen polypeptide, fish protein powder and hydrolyzed animal protein; protein powder include hydrolyzed soybean protein, hemoglobin,hemocrystallin and food-grade whey protein.

目前的已经开发的产品有医药中间体：原卟啉、卟啉铁、蛋白胨、植物蛋白胨；调味品原料：水解植物蛋白粉、肉味蛋白粉；食品原料：复合氨基酸、甘氨酸、大豆缩氨酸、大豆多肽、血肽、胶原多肽、鱼蛋白粉、水解动物蛋白粉和蛋白质粉系列产品：饮料专用型蛋白质粉、血红蛋白质粉、肉制品专用型蛋白质粉。进口的产品有乳清蛋白粉。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P＜0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P＜0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示：(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性，而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P＜0.05)；三组动物的支气管和肺组织常规细菌培养结果均为阴性；感染组动物透射电镜检查见肺泡间隔增宽，其中有较多胶原纤维堆积，其余两组则未见明显异常。

Lavement of pulmonary alveolus of right lung bronchus, undertake left lung of; of cellular classification, computation organizes divide evenly oar to determine group of immunity of collagen content; changes a law to determine sex of STAT1, plaque source grows factor the expression of albumen of 1(PAI-1 of depressor of content of activation of PDGF and fine dissolve zymogen.

右肺支气管肺泡灌洗，进行细胞分类、计数；左肺组织匀浆测定胶原含量；免疫组化法测定STAT1、血小板源性生长因子和纤溶酶原激活物抑制剂1(PAI-1)蛋白的表达。

Results Fibroblast ceils were divided into three groups, and cell proliferation was inhibited in the low-dose group, but promoted in the middle-dose group, while the collagen synthesis was inhibited in the middle-dose group, but pro- moted in the high-dose group.

结果激光照射剂量由低至高可分为3个剂量段，激光强度在第一剂量段时的生物调节效应主要表现为抑制细胞增殖，在第二剂黾段时则能促进细胞增殖并抑制胶原合成，在第三剂量段时间则可促进腔原合成，且每个剂量段内的激光生物调节效应与照射剂量间大致单线性相关。

Cardiac hypertrophy and extracellular matrix remodeling happened as a result of chronic overrange of pressure in heart and hypervolaemia which lead to damage of relaxation and compliance of heart.Drugs including renin-angiotensin converting enzyme inhibitor,angiotensin receptor blocker,aderergic receptor blocker,aldrosteron antagonism,tatins endothelin receptor blocker pancreatic kiniogenase and calcalium channel blocker,can delay or reverse the remodeling by several mechanisms.

主要表现为心脏在慢性超负荷的压力、容量作用下发生的病理改变致使左室肥厚组织胶原结构发生改变，导致心肌顺应性降低，舒张功能受到损害；作用于肾素-血管紧张素系统的药物及肾上腺素受体阻滞剂、醛固酮拮抗剂、他汀类药物、内皮素受体拮抗剂、胰激肽原酶、钙通道阻滞剂等药物通过不同途径和机制延缓和逆转左心室重构。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

Tendon cells 5 x 10(4/ml derived from phalangeal flexor tendon of SD rats were co-culture with CPPF scaffold or CPPF scaffold resurfaced with collagen type-I within 1 week. The co-cultured specimens were examined under optical and electric scanning microscope.

将体外培养 SD大鼠原代趾屈肌腱细胞，以 5×10 6/ ml浓度与 CPPF辫状支架或型胶原涂层的 CPPF辫状支架进行体外混合培养，组织学观察材料与细胞的吸附情况。

Results The BMC surrounding tumor mass and strands showed multi-layered or dissolution, pseudopodia were seen extended from tumor cells, and contained numerous microvesicles.

瘤细胞伸出伪足样突起，且胞浆内含大量微泡，该处基膜消失，胶原原纤维溶解，肿瘤细胞围绕血管部位的血管基膜增厚，纤维组织增生。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。