胶原

The results are as follows: 1The collagenous fibers are arranged in a crass-crossed network of three dimentions. The network in an area of less than 500 μm is like a small board called a microboard. All microboards anastomose each other, forming a microboard network; 2The so-callcd collagenous fibers or lamellae about 4—9 μm thick under the LM are really th...

结果表明：①胶原纤维排列成三维交叉网状結构，后者在约500μm范围内呈板状，称微板，微板相互吻合成微板网；②在LM下所见的厚约4—9μm的"胶原纤維"或"板层"，实际是微板网的垂直切面，⑧文献中的板层结构TEM照片实为其网状结构的一部分，④在胶原纤维之间有少量分支交错的细小纤维，可能是弹性纤维。

MATERIALS: Composite scaffold was made with collagen/chitosan/β-tricalcium phosphate. Upper layer was mainly collagen/chitosan, and lower layer was mainly collagen/chitosan/β-tricalcium phosphate. The factor of porosity was 90%, with antilinear of 100 μm.

材料：以胶原、壳聚糖、β-磷酸三钙制备复合支架，上层以胶原／壳聚糖为主，下层以胶原／壳聚糖／β-磷酸三钙为主，孔隙率≥90%，孔径100μm。

It includes determination of properties of the acid protease and lipase, application of acid enzymes to processes of bating pickling pelt and wet blue of sheepskins, analysis of activities of collagenase in acid protease and trypsin. Comparison was conducted to acid bating and common bating. Orthogonal experiments were used to determine influence of some factors in acid bating on qualities of leather. Scanning electronic microscope was used to observe separating of collage fibers.

对酸性蛋白酶、酸性脂肪酶的性能进行了测定；将其用于绵羊服装革生产的酸性软化及蓝湿皮软化中，利用正交试验法研究了酸性酶软化各因素对成革质量的影响，并与常规酶软化进行了对比；用扫描电镜观察了酸性酶软化后胶原纤维的分散情况；对酸性蛋白酶的分子量进行了测定，对比了酸性蛋白酶与胰酶中的胶原酶活力，以说明酸性酶软化与胰酶软化对胶原性质的影响。

Type Ⅱ collagen-induced arthritis in mice,which shares similar characteristics with rheumatoid arthritis,was used as the mode for RA in this study,by taking the incidence,arthritis index,the level of anti-collagenase Ⅱ antibody,the histo-pathological changes as quotas,we observed cantharis plaster's effect on the model by applicating it on acupoints,and measured the splenic interleukin-1 β level of collagen-immunized mice.

采用与类风湿性关节炎特征相似的小鼠胶原诱导性关节炎(Collagen-induced arthritis，CIA)模型，以关节炎发病率、关节炎指数、抗Ⅱ型胶原抗体水平、组织病理学改变等为指标，观察了斑蝥膏穴位敷贴对此模型的影响，并检测了胶原免疫小鼠脾脏白介素-1β水平。

Abnormal expression of type Ⅰ～Ⅵ could be found in the onset of illness. The decreasing of collagen could causes the weaken of the stability of stroma lamellar and the cornea shap to conus. The onset of keratoconus is related to collagen

胶原Ⅰ～Ⅵ型的表达在圆锥角膜的发病过程中有明显的变化，胶原成分的减少会导致角膜的机械抵抗力减弱，并使角膜前凸变薄，最终影响角膜的正常形态，圆锥角膜的发病与胶原密切相关。

Based on present application status of waste products in curriery in China, the advantages and disadvantages of collagen and analysis and characterization of collagen extracted from hideoffals, studies on collagen application properties and its blend films with one or two polysaccharides were done in this thesis.

本论文基于我国制革废弃物的应用研究现状及胶原蛋白的性能优势与缺点，在减轻制革污染的同时，变废为宝，以高值转化为目的，在对从制革皮边角余料中提取的胶原蛋白进行分析表征的基础上，围绕胶原蛋白的应用特性和共混生物膜材料进行了研究。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

First, with the gelation property of alginate molecule in Ca2+ solution, collagen/alginate threads were prepared through thermally controlled syringing collagen/alginate (w/w=1:3) solution into 2% CaCl2 solution.

第一阶段利用褐藻酸高分子在钙离子水溶液的环境下迅速成胶的特性，再运用湿式纺织技术，将胶原蛋白与褐藻酸混合溶液(胶原蛋白重：褐藻酸重＝1：3)经由恒温注射针筒系统以2ml/min的流速挤压至氯化钙水溶液(2%)的成型槽中，并控制罗拉转速，制备胶原蛋白/褐藻酸细丝。

With collagen gel treatment,Ⅱ°bedsores healed in 2 or 3 weeks,and Ⅲ°bedsores resumed obviously in 4 weeks(60%).

Ⅰ型胶原可促进肉芽生长及上皮再生，以Ⅰ型胶原为主要原料的胶原凝胶能明显缩短褥疮的治疗周期，是促进伤口愈合的良好生物材料，其成份、药效、毒理等已经各种实验验证，值得在临床上广泛应用。

In order to explore application of collagen from rat rail tendon to experimental medicines,the collagen,extracted from solvating fresh rat rail tendons by using a simple economical method,has been prepared collagen gel.

周志瑜 ，廖小宜，余海为了探索鼠尾肌腱胶原蛋白在实验医学中的应用，用简单的方法溶解新鲜大白鼠鼠尾肌腱，从中提取较纯的胶原蛋白，制成胶原凝胶。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。