胶原

BACKGROUND: It is generally accepted that fibrous collagen protein is an ideal vector, limiting by easy fluxion, rapid degradation, and doubtable permeability.

背景：纤维胶原蛋白被公认是非常理想的载体，但其胶性易流动，降解较快，且通透性有待置疑。

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

Ingredients: Hamamelis extracts, plant collagen protein, natural propolis, vitamin E

主要成份：金缕梅衍生物、植物胶原蛋白、天然蜂胶、维生素E等。

A preparation method,using MgO as hydrolyte to isolate collagen protein,the most composition in the leather solid waste,has been developed. A systematic analysis of the hydrolysate has been performed by AFM,SDS-PAGE and amino acid composition analyzer.

首次用AMF、SDS凝胶电泳、氨基酸分析仪等方法，对采用MgO从皮革固体废弃物中提出的胶原蛋白水解产物进行了分析。

A preparation method, using MgO as hydrolyte to isolate collagenprotein, the most composition in the leather solid waste, has beendeveloped. A systematic analysis of the hydrolysate has been performedby AFM, SDS-PAGE and amino acid composition analyzer.

首次用AMF、SDS凝胶电泳、氨基酸分析仪等方法，对采用MgO从皮革固体废弃物中提出的胶原蛋白水解产物进行了分析。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子，本研究构建了带报告基因的载体并检测了其启动子，增强子和减弱子的功能，以此为基础，进一步构建了MBP异位表达载体，为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

SEM result showed that the sample had uniform phase distribution and smooth surface at dried state as well as interpenetrate network structure at hydrogel state. It was seen from the swell ing behavior curves that groups T1, T2 and T3 swelled rapidly within 10-30 minutes, and formed elastic composite hydrogel pipes. In addition, the expanding ratio of inner and outer diameter of the tube was 20%-30% and 100%-120% with swell ing, respectively. The equil ibrium water content of the hydrogel pipes increased with increase of collagen composition. Three groups of samples were immerged in PBS solution contained 2 mg/mL lysozyme at 37 for 1 month, their nozzle cracked, their wall became thin and more transparent. And also, there was small floc deposited on the tube surface.

扫描电镜观察到液氮脆断的人工泪小管内部成分分布均匀，内外壁表面平整，冷冻干燥的人工泪小管断口呈凝胶态的互穿网络结构。3 组不同成分的人工泪小管在PBS 溶液中浸泡30 min 后均快速吸水溶胀，外径扩大100%～ 120%，内径扩大20%～ 30%，并且随胶原含量增高，溶胀速度增快，平衡溶胀率增大。3 组人工泪小管在37℃含2 mg/mL 溶菌酶的PBS 液中浸泡1 个月后，表面有部分细小絮状物，管口开裂，管壁变薄，透明度增加。

Such kind of collagen membrane can accelerate the new bone regeneration in vivo.

以纤维蛋白凝胶为rhBMP-2载体，方法简便可行，并且可以在胶原膜上定量定向复合rhBMP-2。

G. polyhydroxyethyl methacrylate and polyvinyl alcohol. Some potential development and application of the materials are discussed tentatively.

胶原蛋白和一些合成高分子化合物(如聚甲基丙烯酸羟乙酯，聚乙烯醇等)，作为生物材料已得到广泛的应用，而两者形成的复合材料水凝胶具有良好的生物相容性和理化性质，是一类新型的生物材料，具有很大的研究和应用价值。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。