胶原

A 46-year-old man presented with a gradually growing nodule on the left dorsal foot, which had been present for over 10 years. In the beginning, the lesion was treated as a keloid. Because of poor response to treatment, biopsy was arranged. Sections showed a dermal tumor composed of numerous foamy cells, hyalinized wiry collagen, spindle cells and entrapped collagen. The foamy cells were positive for KP1(CD68) and negative for S-100 protein and HMB-45. The histological and immunohistochemical results were consistent with the diagnosis of lipidized fibrous histiocytoma.

一位46岁男性从十年前开始於左足背上出现一个逐渐长大之结节，一开始被当作蟹足肿给予治疗，因为对治疗的反应不好，故安排了切片检查，病理显示一个由大量泡沫状细胞，坚硬而透明之胶原纤维，梭状细胞，胶原纤维束所构成的真皮肿瘤，免疫组织化学染色显示KP1(CD68)阳性，S-100 protein与HMB45阴性，综合病理与免疫组织化学染色结果，诊断为脂肪性纤维组织细胞瘤。

Type II hydrolyzed collagen is made from cartilage.

II 型胶原蛋白专指由软骨细胞产生的胶原蛋白。

By controlling different hydrolyzing condition, collagen hydrolysate with different molecular weight distribution could be achieved for different use.

胶原水解产物的用途不同，对分子量的要求也不同。而目前，对胶原水解产物分子量的测定问题，尚未得到真正

The sham-operation and model groups were redivided into 5 subgroups(6,12 h,1,2,and 4 days) according to the time that the rats were sacrificed;the treatment group were redivided into ...

模型组与治疗组均采用Ⅶ型胶原酶定向注入诱导脑出血模型，假手术组以等量等渗盐水代替Ⅶ型胶原酶定向注入；治疗组给予二异丙酚100 mg/kg腹腔注射，1次/d，假手术组和模型组以等渗盐水代替二异丙酚腹腔注射。

In carcinomatous mucosa, immunoreaction of the carinomatous cell nest was negative, or only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.

结果 正常组织的基底膜呈现很强的Ⅳ型胶原抗体阳性反应，在基底膜形成较粗的环形染色带；Ⅲ型胶原抗体阳性反应在细胞外间质呈连续的细丝纤维样。

Results In increasing dose group, the death rate and the heart weight were increased but the cardiac function were decreased.

结果增量感染组小鼠死亡率持续增高，心脏重量增加，心功能下降，心肌胶原容积分数和血清sIL-2R含量高于其它各组（P〈0.05），病理学特征为基质胶原明显增生重建。

After washed with double-distilled water (10min x 5times), the scaffold were freeze-dried again to obtain the EDAC/NHS treated collagen/chitosan scaffold. The scaffolds were immersed in 75% ethanol for over night for sterilization, followed with solvent exchange by PBS for 5 times at least.

将此种多孔支架置于75％乙醇中浸泡消毒过夜，磷酸盐缓冲液反复漂洗5次以上；将配好浓度的纤维蛋白原溶液以50μl／cm~2的量均匀涂覆在其表面，再以等量的凝血酶滴加在其上，室温或37℃温箱静置30分钟，获得胶原-壳聚糖／纤维蛋白胶不对称支架。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

Methods Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type Ⅰ collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde.

取新鲜成年牛跟腱，用乙酸溶解－酶消化法提取Ⅰ型胶原，并进行盐析、透析、纯化得到精制的液态胶原，用戊二醛进行交联。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。