胶原

The HE and the VG stain were pathologically examined and the expression of TGF-β1 and TNF-α mRNA in the lung tissues were analyzed by nucleic acid hybridization in situ. Results: YQHX1,2,3 obviously alleviated rats alveolitis and fibrosis.

结果：模型组肺泡炎和肺纤维化程度较重，且其胶原纤维沉积较多，TGF-β1、TNF-α的表达增多；而中药治疗组肺泡炎和纤维化程度较轻，胶原沉积明显减少，TGF-β1、TNF-α表达减少。

The proliferation of medial smooth muscle cells and collagenous fibers of pulmonary arteriole in patients with PH were observed by electron microscopy.

电镜下，中重度PH组肺细小动脉中膜平滑肌增生，外膜胶原纤维密集；轻度PH组肺细小动脉中膜平滑肌细胞和外膜胶原纤维增生轻于中重度PH组；无PH组肺细小动脉结构改变不明显。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组，给药组涂抹红鹿制剂，对照组涂抹化妆品基质，连续涂抹60 d；应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

DESPRIPTION】 This product adopts natural activated Collagen Peptide as its ingredient, and combines the SAYF(skin activate and youthful factor) and micro spinning technology of elastic fiber to make the eye pact perfectly fit around the eye contour, It can quickly supplement skin moisture and provide quick penetration of nutrient substance to the stratum basale under the guide of the nutrient substance penetrating absorption principle, as well as promote the vitality of skin cells, 95% of which will efficiently absorb and reserve moisture and balance PH value to make the skin elastic and soft, and significantly improve low collagen synthesis which is related to aging. It can also efficiently prevent and reduce eyes' skin wrinkles and cutis laxa to restore the eyes' skin moist and elastic, and implement the nutrition lost because of the fluctuations in temperatures to promote the natural moisture ability of the skin around the eyes to make those skin instantly moisturized, smooth, refresh and outstanding radiant. The wrinkles and fine lines will be reduced at the same time, and the black circles will be no longer apparent.

本品采用天然的活性胶原蛋白多肽为原料，独有的SAYF融合弹性纤维的微纺技术，完美贴合眼部轮廓，可快速补充皮肤在外界所流失的水分，功效营养物质通过透皮吸收原理，快速渗透到皮肤的基底层，并渗提高皮肤细胞活力，其95%有效吸收留住水分，平衡PH值，使皮肤增加弹性和柔软性；明显改善与老化相关的胶原合成低下的现象，有效预防和减少眼部肌肤皱纹及松弛的现象，恢复润泽和弹性，同时补充眼部皮肤在各种温度不定的情况下流失的营养，提升眼周肌肤自然储水力，双眸瞬间滋润、光滑、丰盈，焕然一新，细纹与皱纹减少，黑眼圈变得不再明显。

The methodology and its mechanism for the biomimetic synthesis of HAp/Col bone-like composite by in vitro imitating bone biomineralization and self-assemble process of materials is reviewed here. hydroxyapatite/collagen ; bonelike composite ; biomineralization ; self-assembly

从成分上来讲，天然骨除了含有羟基磷灰石无机成分外，还含有胶原(collagen，Col)、糖蛋白、糖胺聚糖等少量的有机成分无机物与有机物含量之比约为7∶3，其中胶原占有机质重量的90%犤2

Transmission electron microscopy examination: epithelium cells degeneration, chromatin margination and collagen arrangement disorder could be observed at early stage after bothsided LASIK.

透射电镜下：BSL术后早期上皮细胞轻微变性，少量纤维细胞核染色质边集，角膜瓣边缘局部胶原排列稍紊乱。1mo时基质胶原纤维排列整齐，直径大小一致。

Three hydroxyapatite powders (HAP1, HAP2 and HAP3) were prepared by hydrolyzation process using brushite, precipitation method using calcium hydroxide and ammonium phosphate and neutralization method using calcium hydroxide and phosphoric acid, respectively. Hydroxyapatite/collagen composite was prepared by in situ precipitation method using calcium hydroxide, phosphoric acid and bovine collagen which was extracted from the fresh bovine tendons.

本研究以相关钙盐、磷酸盐为原料，分别用二水合磷酸氢钙水解法、氢氧化钙-磷酸氢二铵沉淀法和氢氧化钙-磷酸酸碱中和工艺制备了三种羟基磷灰石粉体，分别为HAP1、HAP2和HAP3；并以氢氧化钙、磷酸和从牛腱中提取的胶原为原料通过原位沉淀制备了羟基磷灰石/胶原复合材料。

Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope.

将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上；MTT法检测支架对人牙周膜细胞黏附、生长的影响；并用倒置相差显微镜和扫描电镜观察形态变化。

METHODS: The collagen type Ⅰ gene was cloned by the improved subtractive hybridization, and the fragment of collagen type Ⅰ cDNA was labeled by digoxin and was hybrided by in situ hybridization on the section of capsule of normal and cataractous lens.

用改良的消减杂交法克隆Ⅰ型胶原基因，地高辛标记Ⅰ型胶原cDNA片段制作探针，用原位杂交法研究其在晶状体前囊膜切片上的表达，并作统计学处理。

METHODS: The collagen type Ⅰ gene was cloned by the improved subtractive hybridization, and the fragment of collagen type Ⅰ cDNA was labeled by digoxin and was hybrided by in situ hybridization on the section of capsule of normal and cataractous lens.

用改良的消减杂交法克隆Ⅰ型胶原基因，地高辛标记Ⅰ型胶原cDN####段制作探针，用原位杂交法研究其在晶状体前囊膜切片上的表达，并作统计学处理。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。