胶原

The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%，主要为是I型、III型胶原蛋白，本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白，将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解，液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽，利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

Small intestinal biopsies showed subepithelial collagen deposition with varying degrees of villous atrophy and varying numbers of intraepithelial lymphocytes. Four patients had previous biopsies showing enteropathic changes without collagen deposition. Seven cases were associated with collagenous colitis and 1 also had features of lymphocytic colitis. Three patients also had collagen deposition in gastric biopsies. One case was associated with lymphocytic gastritis. Celiac disease (CD, gluten-sensitive enteropathy) was documented in 4 patients. Five patients made a clinical improvement with combinations of a gluten-free diet and immunosuppressive therapy. Two patients died of complications of malnutrition and 1 of another illness. Clonal T-cell populations were identified in 5 of 6 cases tested. Four of these patients improved clinically after treatment but 1 has died. Collagenous sprue evolved on a background of CD in 4 cases. There was no history of CD in others and these cases may be the result of a biologic insult other than gluten sensitivity.

小肠活检表现为上皮下胶原沉积，绒毛萎缩程度不一，上皮内淋巴细胞浸润数量不等。4例患者以往曾做过活检，表现为其他肠病改变，并没有胶原沉积。7例患者合并胶原性结肠炎，且1例还有淋巴细胞性结肠炎的特征。3例患者胃活检也发现有胶原沉积。1例患者合并淋巴细胞性胃炎。4例患者有乳糜泻（CD，谷蛋白敏感性肠病）病史。5例患者经无麸质饮食和免疫抑制治疗后，临床症状有所改善。2例患者因营养不良合并另一种疾病而死亡。6例患者做了T细胞受体基因重排，其中5例发现有克隆性T细胞群，这5例中有4例治疗后临床症状加重，且1例死亡。4例患者在CD的基础上病发胶原性口炎性腹泻；其余患者无CD病史，他们的发病也许是生物源性损害，而非谷蛋白敏感。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果：提取液主要为Ⅰ型胶原蛋白；72小时37℃恒温培养箱内培养无细菌生长；紫外分光光度计测定，吸收高峰为230nm；氨基酸成分主要为甘氨酸：占40%，脯氨酸：14.3%。显微镜下见：相互交织的胶原原纤维结构组成的网状结构；纤维均匀，有规则横纹。胶原蛋白液在37℃恒温下，可制成凝胶，用于培养细胞；用磷酸液冲洗胶原蛋白凝胶可成固体片状，制成胶原膜，用作医用材料。

Myofibril fragmentation index, contents of collagen (total collagen, heat soluble collagen, heat insoluble collagen) and muscle fiber diameter were used to evaluate the histology characteristics, and meat tenderness of Chinese mitten crab from 4 different locations was analyzed by using the method of combination index.

中文摘要：摘要：采用肌原纤维小片化指数、胶原蛋白（总胶原蛋白、热溶性胶原蛋白、热残留胶原蛋白）和肌纤维直径3种评定方法，分别测定了4地区中华绒螯蟹肌肉的组织特性，并初步探讨了采用综合指数法比较肉质的嫩度。

Collagenase activity was defined as the radioactivity extractable by 50% dioxane. Clostridial collagenase was used as a standard. The lower limit of this assay method was 0.01 units of clostridial collagenase. Among 17 glaucoma eyes, 6 had trace collagenase activity (around 0.01 units) in aqueous humor; and for cataract eyes, 8 out of 27 had trace collagenase acitivty.

我们所采用之测量方法可侦测出约0.01单位以上的胶原酵素；一般前房液之胶原酵含量非常低，许多患者无法以我们之方法利得胶原酵素之存在：17位青光眼患者中，只有6位可於其前房液中到得约0.01单往之胶原酵素；27位白内障急者中，有8位可测得微量胶原酵素；二者在统计学上与太大差异存在。

Want the skin to still can produce new collagen only, cuticular collagen is enzymatic build in responsible compose crudely collagen while also adjust of skin collagen form; Cut generation collagen is enzymatic.

只要皮肤还能产生新的胶原质，表皮胶原酶就在负责构建未成熟的胶原质的同时也调节皮肤胶原质的形成；伤口产生胶原酶。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后，胶原排列趋于一致，微血管丰富而呈开放状态，成纤维细胞的前胶原聚合体蓄积在细胞浆内；胶原总的含量减少，但Ⅲ型前胶原含量维持在一个较高水平；透明质酸含量逐渐升高，接近正常皮肤水平。

Methods Constructed the model of fetal scarless wound healing and investigated the expression of collagen in fetal and adult rat wounds by immunohistochemistry and in situ hybridization.

染色，Ⅰ、Ⅲ型胶原免疫组化，Ⅰ、Ⅲ型胶原原位杂交。结果胎鼠伤口愈合快，组织结构再生完全，没有瘢痕形成，胶原呈网状正常排列，Ⅰ、Ⅲ型胶原蛋白及表达均增多，同时伴细胞数增多，Ⅲ型胶原首先形成网状排列；成年伤口胶原纤维沉积慢，平行排列的胶原逐渐呈束状填塞伤口以致瘢痕形成，伤口内Ⅰ、Ⅲ型前胶原mRNA表达均增多，细胞数目增多不明显。

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性，TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase，expression of TIMP-1，and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的：研究尿道瘢痕的细胞外基质的组成，尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响；比较胶原酶活性，金属蛋白酶组织抑制因子-1（TIMP-1）以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异；研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

It is well known that the ordered triple helical structure of collagen is stabilized by both interchain hydrogen bonds and "structural" water molecules. But from the point of application, the use of plain native collagen as materials is often precluded by inadequate physical-chemical and mechanical properties.

胶原蛋白的保湿性能特别优异，用胶原蛋白纺丝得到的胶原蛋白纤维所制成的纺织面料和衣服将保留一部分天然胶原蛋白的性能，且胶原蛋白的结构与人体皮肤具有相似性，因而与人体皮肤具有较好的亲和性，穿着更加舒适。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。