胶化体

The method includes the following steps: a semiconductor basal body is provided and the insulating layer of the semiconductor basal body is plated with light-sensitive lacquer and then is exposed in light and at last is developed; etching process is carried out to make a through hole on the insulating layer; cineration process is done, the remanent light-sensitive lacquer is removed and the temperature for the cineration process is 20-50 DEG C; wet cleaning process is carried out.

该制造方法包括如下步骤：提供半导体基体，对半导体基体的绝缘层进行镀光刻胶步骤、曝光步骤、显影步骤；进行蚀刻步骤，在绝缘层上形成通孔；进行灰化步骤，将剩余的光刻胶移除，且灰化步骤的温度条件范围是20-50摄氏度；进行湿法清洗步骤。

It was found that there were 36 distinct differences in 184 protein spots. In-gel digestion and liquid chromatography mass spectrometry C LC-MS were made to analyze the protein SSP2801, and Mascot software was applied to identify the protein. The results showed the protein was large subunit of Rubisco, and its content in yellow mutant decreased distinctly, only about 26% of the comparison. Maybe it has relation to etiolation.

切耿其中差异最为明显的SSP2801位点蛋白进行胶内酶切、LC-MS分析，并应用Mascot软件检索鉴定，结果表明该蛋白组分为RUBP羧化酶的大亚基，其在突变体中含量明显减少，仅为对照的26％，表明突变体叶色黄化与RUBP羧化酶大亚基含量减少也有关系。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

The psammoma body mineralization in meningioma is a common type of mineralizationThe analysis of the mineral composition may provide some support information in finding the reason of happening and developing of the diseaseThis paper focuses on the concentric layered structure mineralization in meningiomas, using mineralogical methods, such as HRSEM, ESEM, EDAX, EPMA, HRTEM, XRD and FTIR to systematically investigate the mineral composition, structure and shape of the minerals in psammoma bodies in meningiomasWe have devised a method for preparing the silicon wafer sheet which was used for the ESEM insitu observations and analysisIn this study, we first got the ESEM and HRTEM images of the initial mineralization phase of meningiomasThese images showed that in the early stage of psammoma body mineralization in meningiomas, many mineralized balls composed of octocaphosphate were precipitated on the collagen fibersThese balls continued to grow and aggregate, and were gradually hydrolyzed to become the dahlliteThe continued development of mineralization resulted in the mineralized collagen fibersThe study revealed that the concentric layered structure of the psammoma bodies in meningiomas is formed by the spiral arrangement of the mineralized collagen fibers on which the mineralized grains precipitated.

砂粒体矿化是脑膜瘤中常见的矿化类型，对其形成机理和矿物成分的分析可能会对肿瘤发生、发展的研究提供辅助信息。该研究选取人脑膜瘤中的砂粒体矿化作为研究对象，采用偏光显微镜、环境扫描电镜及能谱、X射线衍射仪、高分辨透射电镜和电子探针对样品的形貌、结构和成分进行测试分析，并以此为依据探讨脑膜瘤中砂粒体的形成机理。研究结果表明矿化的初期为沉淀在胶原纤维上的矿化小球，成分为磷酸八钙；矿化小球不断生长聚集，并逐步水解为碳羟磷灰石晶体，矿化的不断发展致使胶原纤维也发生矿化。砂粒体的同心层状构造是由螺旋状排列的矿化胶原纤维及沉淀在其上的矿化颗粒组成的集合体，而不是多数研究中所述：砂粒体是以坏死细胞残骸为中心由内至外的同心层沉淀。

"Tell us about it, Janet," Gel Sadra and Dr. Raphael cackled.

&告诉我们关於它，珍妮&，胶化体 Sadra 和被咯咯地叫的拉斐尔博士。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

For the case of pH-induced micellization, as pH of the solutions decreases to below 4.6, micellar structure having insoluble complexes of PAA/PNiPAAm as the core and solvated HEC chains as the shell can be obtained due to hydrogen-bonding complexation between the proton-acceptors in PNiPAAm and the proton-donors in PAA.

首先发现pH诱导胶束形成的临界pH值在4.6附近，证实了胶束化机理是由于低pH下PAA接枝链上的质子给体与PNiPAAm支链上的质子受体间的氢键络合形成胶束的&核&，HEC不参与络合形成胶束的&壳&。

In this paper, the authors found the pyritized rod-like bacteria in desmocollinite and the Cyanophytes's gelationous sheath and the degraded organic matter of blue green algae in collinite of high-sulfur coal seams No.9 w(So=2.67% and No.10 w(So=2.54% using SEM-EDX and TEM. These findings of bacteria and algae suggest that they play an important role in the high-sulfur formation.

应用带能谱仪的扫描电镜和透射电镜，在乌达矿区高硫煤9煤层和10煤层的基质镜质体中发现了黄铁矿化的杆状菌落，在无结构镜质体中发现了蓝藻胶鞘及其降解有机质，这些菌藻类体的发现表明低等生源在高硫煤形成过程中起了重要作用。

Raphael and Gel Sadra rising through the trap door in the stage.

帐上涨 ，显示升起过阶段的圈套门的肯恩，六月， Jinpei ，拉斐尔博士和胶化体 Sadra 。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。