胶化

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Result: 1. The method of arc-needle injection with 131I-iodinated oil step-by-step sector-shapely improved obviously the drug distribution, comparing with routine linearity injection. 2. SPECT image reconstruction detect 131I-iodinated oil three-dimensional distribution quantitatively. 131I-iodinated oil metabolism equation may obtained by continuous measurements, in order to calculate absorption dose of p particle at target. 3. ZT glue lengthens obviously 131I-iodinated oil retention in mouse local subcutis and rabbit liver which can rise local radiation effect, decrease injury at normal tissue.

结果：1、弧形针分段扇形注射~(131)I-碘化油，比常规使用的线性注射明显改善药物弥散均匀度。2、SPECT断层扫描定量检测~(131)I-碘化油的三维分布，连续测量建立~(131)I-碘化油的代谢方程，并计算~(131)I对靶区的β辐射贡献的吸收剂量。3、ZT胶明显延长~(131)I-碘化油在大鼠皮下、兔肝脏内的滞留。

The microstructures of the new developed carbon aerogels was investigated by means of low-temperature N_2 adsorption, TEM, XRD, XPS and etc. We studied the influence of synthesis conditions on the aerogels structure, such as reactant mass percent, catalyst concentration, carbonization process and supersonic.

采用低温N_2吸附、TEM、XRD和XPS等手段研究了碳气凝胶的微观结构特点，考察了反应物质量分数、催化剂浓度、碳化过程对气凝胶孔结构的影响，同时还研究了超声作用对气凝胶微观结构的影响。

Selection of our products well, pure texture, the choice of materials are: acrylonitrile-butadiene rubber, silicone, ethylene-propylene rubber, fluoride plastic, hydrogenated nitrile, Poly-resin, folder cloth, rubber, PU expected, polytetrafluoroethylene, nylon, POM, engineering plastics (such as high-strength performance PPS, PEEK) and graphite (such as ordinary graphite, high - Pure graphite impregnated graphite), ceramics, alloys, Pan-gen, synthetic materials, etc.

我们的产品选材精良、质地纯正，选用的材料有：丁腈胶、硅胶、乙丙胶、氟胶、氢化丁腈、聚安脂、夹布、橡塑、PU料、聚四氟乙烯、尼龙、聚甲醛、工程塑料（如高强性能PPS、PEEK等）；及石墨（如普通石墨、高纯石墨、浸渍石墨）、陶瓷、合金、盘根、合成材料等。

Selection of our products well, pure texture, the choice of materials are: acrylonitrile-butadiene rubber, silicone, ethylene-propylene rubber, fluoride plastic, hydrogenated nitrile, poly - Fat, folder cloth, rubber, PU expected, polytetrafluoroethylene, nylon, POM, engineering plastics (such as high-strength performance PPS, PEEK) and graphite (such as ordinary graphite, high-purity graphite impregnated Graphite), ceramics, alloys, Pan-gen, synthetic materials.

我们的产品选材精良、质地纯正，选用的材料有：丁腈胶、硅胶、乙丙胶、氟胶、氢化丁腈、聚安脂、夹布、橡塑、PU料、聚四氟乙烯、尼龙、聚甲醛、工程塑料（如高强性能PPS、PEEK等）；及石墨（如普通石墨、高纯石墨、浸渍石墨）、陶瓷、合金、盘根、合成材料等。

The feasibility analysis on the packer's function of sealing,anchoring and supporting has been launched,and the contact finite element analysis of the middle rubber with four different lengths has been finished,appropriate length of the middle rubber has been got,and a foundation has been laid for analysis of rubber combination.

开展了水平井酸化封隔器实现密封、支撑、锚定功能的可行性分析，完成了四种长度的中胶筒接触有限元分析，得到了合适的中胶筒长度，为组合胶筒地分析奠定了基础。

INGREDIENTS: skim milk, sugar, cream, chocolaty chips (sugar, coconut oil, cocoa processed with alkali, partially hydrogenated coconut oil, cocoa, salt, soy lecithin, natural flavor), corn syrup, cocoa processed with alkali, whey protein, egg yolks, tapioca maltodextrin, soy protein, natural flavor, Propylene Glycol Monostearate, guar gum, carrageenan, cellulose gum, mono and diglycerides, salt, malt extract, xanthan gum, molasses, vitamin A palmitate, dextrose, caramel color

成分：脱脂牛奶，糖，奶油， chocolaty芯片（糖，椰子油，可可碱处理，部分氢化的椰子油，可可粉，盐，酱油，卵磷脂，天然香料），玉米糖浆，可可碱处理，乳清蛋白，蛋蛋黄，木薯麦芽糊精，大豆蛋白，天然香料，丙二醇酯，瓜尔豆胶，卡拉胶，纤维素胶，单声道和diglycerides ，盐，麦芽提取物，黄原胶，糖蜜，维生素A棕榈酸酯，葡萄糖，焦糖色

The optimal experimental conditions for the stability of immobilized xanthan gum were produced at the pH value of 2.0 and temperature 60℃.

在本实验条件下，固定化三仙胶之结构稳定性以凝胶溶液pH为2及凝胶温度60℃为最佳操作条件。

Hydroquinone-formaldehyde organic aerogels with high HC ratio (10~40, molar ratio of hydroquinone to catalyst) have been prepared using sol-gel process. And carbon aerogels have been prepared by pyrolyzing the aerogels.

溶胶－凝胶法制备了高HC比（10~40，对苯二酚与催化剂(Na2CO3)的物质的量之比）的对苯二酚－甲醛有机气凝胶，并经高温碳化处理得到其碳气凝胶。

Our main products are: computer automated rapid freezing and thawing of concrete tester, concrete carbonation chamber, concrete move Miriam, concrete to accelerate the conservation of boxes, concrete mixer, cement mortar conservation tank, cement rapid conservation boxes, cement paste mixer, cement glue sand mixer, cement mortar Tap Taiwan, the electric bending testing machine, making me boil marcescens, electric saws stone machine, sieve analysis of negative pressure device, Rutting Tester, Pavement Deflection Tester, rotating compaction test equipment etc..

公司主要产品有：微机全自动混凝土快速冻融试验仪、混凝土碳化试验箱、混凝土动弹仪、混凝土加速养护箱、混凝土搅拌机、水泥胶砂养护水槽、水泥快速养护箱、水泥净浆搅拌机、水泥胶砂搅拌机、水泥胶砂振实台、电动抗折试验机、雷氏沸煮箱、电动锯石机、负压筛析仪、车辙试验仪、路面弯沉试验仪、旋转压实仪等试验设备。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。