胶凝

This consideration indicates that different inter- molecular forces are responsible for self-assembly and that gelation efficiency is the result of this delicate balance.

这一考虑表明不同的分子间的力对自组装起主导作用，而且凝胶形成效率是这一微妙平衡的结果。

Methods Effect of concentrations of CS and GPS and pH of the mixture solutions on the gelation of CS/GPS solution were investigated.

方法研究不同浓度的壳聚糖和甘油磷酸钠、混合溶液的pH值等因素对CS/GPS溶液凝胶化性能的影响。

The relation between gelation point and gel content of IPNs with different compositions were determined.

丙烯酸甲酯在生成IPN过程中凝胶的生成速度要比苯乙烯的场合快。

However, the gelation tune was decreased with the increase of water amount and increased with the increase of ethanol amount.

在类似"两步法"中，通过同时使用的氢氟酸和氨水，凝胶化时间更短，其最佳条件是无水乙醇和水与正硅酸四乙酯的体积比各自是4和0.6，同时加入氢氟酸和氨水的体积比为1：1时，获得的比表面积最大。

Calcium chloride modified nanoparticles prepared by means of chemistry. Analyse the combination of calcium phosphate nanoparticles and DNA, the protection to DNA as well by gelose gelatin electrophoresis. When the green fluorescence protein gene was regarded as report gene, gene vector of calcium phosphate nanoparticles transfected CNE-2 cell in vitro and vivo. Combine the calcium phosphate nanopartides and suicide gene yCDg1yTK for Nasopharyngeal Carcinoma therapy in vitro.

化学方法制备，氯化钙修饰纳米颗粒，用琼脂糖凝胶电泳分析磷酸钙纳米颗粒与DNA的结合效率及对DNA的保护作用，用绿色荧光蛋白基因作报告基因，将磷酸钙纳米颗粒为基因载体转染鼻咽癌(CNE-2)细胞和在动物体内转染肿瘤细胞；以及将磷酸钙纳米与自杀基因yCDglyTK结合，并在体外对鼻咽癌进行基因治疗。

It was found that TPS4-1 was a polysaccharide-protein-nucleic acid conjugate by using the methods of UV, IR, NMR, HLPC, GC, gelose electrophoresis, periodate oxidation, smith degradation.

通过吸附柱层析、离子交换柱层析和葡聚糖凝胶柱层析等多级层析纯化的方法获得了茶多糖的相对均一组分TPS4-1。

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

猪圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因，也是区分猪圆环病毒1型(PCV1)和猪圆环病毒2型(PCV2)的重要基因，本研究参照GenBank已发表的15株PCV2基因序列，设计合成一对引物，对PCV2的ORF2基因进行PCR扩增，产物经琼脂糖凝胶电泳，呈现一条大小约0.67kb的特异条带，经条件筛选，建立了猪病料中PCV2感染的PCR检测方法。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

objective to compare four postoperative nasal packing materials.methods total 136 patients,80 cases with chronic sinus it is and under went endoscopic sinussurgery,were randomly packed using four different hae mostasis materials.in which,paraffin gauze group was 40 cases,rapid sorbalgon group 27 cases,merocel group 36 cases and gel knit group 31 cases.the packing materials wereemoved after left for 24 hours to 48 hours.the efficacy of nasal packing materials was asassessed interm of the levels of nasal pain or headache in the leaving period and nasal bleeding after removal of nasal packing.results the in cidence rate of nasal pain or headache respectively was 82.5% in paraffin gauze group,44.4% in merocel,11.1% in sorbalgon and 3.2% in gel knit.the significant statistical difference was observedbetween the four groups (χ2=70.21,p.01).the incidence rate of nasal bleeding after removed was 85%,11.1%,41.7% and 9.7% respectively.the statistical difference was also significant (χ2=54.28,p.01).conclusions the choosing of postoperative na sal packing after functional endoscopic sinus sur gey depends on various factors,gel knit has much advantages and can be used as routine packs after fess.

摘 要］目的：比较四种鼻腔填塞材料的疗效，指导临床选择合适的术后鼻内填塞物。方法：对134例慢性鼻窦炎分别采用凡士林纱条(40例)、藻酸钙纤维(sorbalgon，27例)、膨胀海绵(merocel，36例)和瑞纳凝胶快速止血材料(gel knit，31例)四种材料填塞鼻腔，24 h～48 h取出填塞物，根据填塞后鼻腔胀痛、头痛程度，取出填塞物后鼻腔出血程度等评价疗效。结果：凡士林纱条填塞组，82.5%有鼻腔胀痛或头痛；sorbalgon藻酸钙纤维组11.1%；merocel组44.4%；gel knit组3.2%，四组比较差异非常显著(χ2=70.21，p.01)。取出填塞物后鼻腔再出血分别为85%，11.1%，41.7%和9.7%，四组比较差异非常显著(χ2=54.28，p.01)。结论：鼻内镜手术时，应综合考虑诸方面因素合理选用术后填塞物。瑞纳优势较为全面，可作为鼻内镜手术的常规填塞材料。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。