胶凝

The PRD weak gelling spudding fluid is successfully used in NB35-2 Oilfield, by which problems of fluid leaking from shaking screen and viscosity break are solved.

PRD弱凝胶钻开液体系在NB35-2油田的成功试用，解决了传统PRD钻开液在应用中存在的振动筛跑浆和破胶难等问题。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Collagen gel induced cleavage of FAK in various cell types, but did not necessarily induce apoptosis of them.

在不同型态的细胞中发现胶原蛋白凝胶均会造成FAK的分解，因此FAK的分解并非造成凋亡之必要条件。

Objective To assess the effects of curing bedsores with the compound of collagen gel.

目的 探讨胶原凝胶复合物对各种类型褥疮的治疗效果。

The amorphous gelatin was heated by self-combustion in microwave oven. The burning process of amorphous gelatin was studied by TG-DTG. The products were detected by X-Ray and TEM.

利用热分析研究了干凝胶自燃烧过程，并且利用X衍射、透射电子显微镜研究了pH值、温度、成胶时间以及燃烧温度对MnZn铁氧体的晶体结构、晶体组成和粒径大小的关系。

The diffusion and cross—linking of polymer chains for blend latex particles were investigated by measurement of gel content,swell ratio and FTIR.The influences of different core／shell structure of blend latexes on mechanical properties of the films were described.

通过胶膜的凝胶率和膨胀率的测定和红外光谱分析对反应性复合乳液中乳胶粒子的扩散及交联反应进行了研究，并探讨了不同核壳结构复合乳液对涂膜机械性能的影响。

After visible light illumination 4h, the photodegradation ratio of methyl blue reached 75.6％by sol-gel process and 87.5％with reverse micelle method.

可见光照射4h，溶胶-凝胶法和反胶束法制备的4.2％CdS复合TiO_2粉体，光催化降解亚甲基蓝的效率分别达到了75.6％、87.5％。

The gel property of synergistic action between xanthan gum and κ-carrageenan and its application in producing cooked ham was studied.

摘 要 对黄原胶与κ-卡拉胶复配的凝胶特性及在蒸煮火腿中的应用进行了研究。

A CEVd strain was isolated from Heibei province in China and its complete nucleotide sequences were identified.Accounting to the characteristic of structure of viroid, the nucleic acid from the positive material was detected by bidirectional PAGE.

柑桔裂皮类病毒快速检测技术体系的建立聚丙烯酰胺双向凝胶电泳法检测：将从阳性材料上提取的类病毒核酸，先在6％的聚丙烯酰胺非变性胶上进行第一向电泳，然后切割含类病毒分子的胶条，在变性条件下进行垂直或反向电泳，最后银染观察。

These results indicated that activation of proteases and protein synthesis inhibition play important roles in collagen gel-induced apoptosis.

由上述结果指出，蛋白酵素的活化在胶原蛋白凝胶诱发的细胞凋亡过程扮演重要角色。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！