胶凝

The extent and velocity of hydrating and swelling of matrix were affected by the pH of the dissolution medium and period of time.

HPMC水凝胶的形成速度和形态与介质的pH有关，凝胶层的溶蚀速度控制药物的释放。

MethodsThe rat hind paw swelling elicited by injection of carrageenan and measurement of NO concentration were used to evaluate anti-inflammatory activities; Analgesic effects were evaluated by torsion body method; expandedass and capillary tube methods were used to detect cruor activity.

方法采用角叉菜胶致大鼠足跖肿胀模型和检测NO含量评价延龄草乙醇提取物的抗炎活性；用扭体法观察延龄草乙醇提取物的镇痛作用；采用玻片法、毛细管法测凝血时间研究延龄草乙醇提取物的凝血作用。

The crystallizability and solution properties of polyacrylate depressants and their depressive effects on two waxy oils were studied. It was found that the crystallizability of PA depended on the length of its side chain and molecular weight. Gel was observed in the concentrated solution, The temperature of gelation, which was a measure of the crystallizability of PA in solution, was higher for the PA itself with stronger crystallizability.

对聚丙烯酸长链酯的结晶能力和溶液性质及其对两种含蜡油的降凝效果进行了研究，发现PA的结晶能力取决于PA侧链长度和相对分子质量，而其在溶液中形成冻胶或沉淀的温度（反映PA在溶液中的结晶能力）与PA本体的结晶能力有关，而PA对含蜡油的降凝效果则与PA在油中的结晶能力有关。

The castor oil based polyurethane potting materials were synthesized from castor oil,4,4 diphenylmethane diisocyanate,trimethylolpropane and 1,2 propanediol.

以蓖麻油，4，4' -二苯基甲烷二异氰酸酯、三羟甲基丙烷与 1，2 -丙二醇为原料合成了蓖麻油基聚氨酯灌封胶，研究了灌封胶的固化流变行为和凝胶化时间。

Chitosan-Collagen-GAGs collagen gelart ificial skin is a new collagen gel living artificial skin which has certain antibacterial ability and stratified epiderm is and dense dermis structure like normal skin.

壳多糖-胶原-GAGs胶原凝胶人工皮肤是一种新型、有一定抗感染能力的活人工皮肤。

Moreover, the A-B-A triblock copolymer solution in a selective solvent, which is good for the block B, was explored. It was observed that the flowerlike micelles were formed, in which the central block B took on a loop conformation and the two end A blocks became a part of the micellar core. It was found that some of the soluble middle B blocks served as extended bridges between the small clusters by the poorly solvated end A blocks.

在A-B-A三嵌段共聚物溶液体系中，溶剂是链段B的良溶剂，是链段A的不良溶剂，此时我们得到了花型结构的胶束，其中花心是由链段A聚集而成，花瓣是由链段B形成的，而且在体系中也发现了有些胶束间存在着桥，并且在浓度很高时，体系出现了凝胶现象。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Three kinds of molecular weight determination methods, i.e., size exclusition chromatography, sodium dodecyl sulphate-polyacrylamide gel electro phoresis and high performance liquid chromatography / electro spraying ionization-mass spectrometry (HPLC/ESI-MS) are introduced to study of the attribute of collagen hydrolysates.

应用体积排阻色谱、聚丙烯酰胺凝胶电泳、高效液相与电喷雾质谱联用(HPLC/ESI-MS)这三种蛋白质分子量分析测试技术，来表征胶原水解产物的分子量，以寻求较好的胶原水解产物分子量的测试方法

The knowledge obtained here as well as the new morphologies identified provide useful information for future rational design and synthesis of novel multicompartment micelles.5. A superstrong segregation regime theory model for core-corona micelles and core-shell-corona micelles is developed.

这些信息对将来合理设计和合成新型多隔段胶束提供了指导。5、建立了用于描述核-冠胶束和核-壳-冠状胶束的超强分凝形式理论模型。

Results The maximum absorbance of the purified collagen appeared at 230nm, with correspondence to type Ⅰ collagen, two close bands on SDS-PAGE (mol. wt 98 000) suggested the purified product was type Ⅰ collagen.

结果：提取的胶原紫外分光光谱最高吸收峰值为230nm左右；聚丙酰胺凝胶电泳呈现2条相邻带（相对分子质量98000左右），符合Ⅰ型胶原的特性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！