胶凝

For decreasing the quantity of the organic solvent and saving the environment, this paper is focused on the preparation of stable TiO2 sol by using titanium alkoxide as precursor and water as solvent while adding acetic acid for the purpose of moderating the rate of hydrolysis.

溶胶-凝胶处理可以在织物的表面形成复杂的氧化物网络结构，这层凝胶固着在织物的表面，改表纤维的表面化学性能，赋予织物一定的功能性。

To assess the efficacy of resiquimod 0.01% gel for reducing human anogenital HS-2 mucosal reactiation, the researchers studied 75 subjects with genital HS-2 who were randomized to apply the agent or the ehicle gel alone to herpes lesions twice weekly for 3 weeks.

评价0.01%雷西莫特凝胶减少人肛门生殖器HS-2粘膜复发的功效，研究者研究了75例生殖器HS-2感染者，随机给予雷西莫特口服或每周两次疱疹皮损处单独应用雷西莫特凝胶，连用三周。

Methods: During the April 2003 to December 2004, from the patients with different position colorectal cancer beyond Dukes C stage who were performed laparoscopic radical operation random draw 20 patients as experiment group, and from patients with different position colorectal cancer beyond Dukes C patients who were performed open radical operation 20 patients took randomly as contrast group. We draw thepatients blood about 5 ml from antecubital vein at the time before and after the operation about 30 minutes respectively, preserved the blood in deep hypothermia circumstance,cytrokeratin-20(CK-20) as the micrometastasis maker, using the RT-PCR technique,G3PDH as the intra-contrast gene, quantitatively detect the amounts of CK-20 in the blood with the gelatum imagery system and the computer analyzing software on the gelatum imagery system ,in order to compare the influence of the laparoscopic and the open operation on the micrometastasis.

在我院2003年4月—2004年12月间不同部位的临床分期在Dukes C期之内的接受了腹腔镜结直肠癌根治术结直肠癌患者中随机抽取20例作为试验组，另外在同期的接受开腹手术患者中随机抽取20例作为对照组，分别抽取术前30min和术后30min的肘前静脉血5ml，集中低温保存，以细胞角蛋白(cytekeratin-20)CK-20为微转移的标志物，应用RT-PCR技术，以G3PDH为内对照基因，应用凝胶成像系统及其计算机凝胶成像系统分析软件来定量检测手术前、后患者外周静脉血中CK-20含量，比较腹腔镜和开腹的手术方式对肿瘤微转移的影响。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果：得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41；组内的平均匹配率分别为91.6%，90.20%对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

Discussion on membrane formation mechanismBased on the Flory-Huggins theory, Phase equilibrium equations of PVDF/DMAc/water ternary system were wrote out, and the binodal and spinodal lines in the phase diagrams of the system were obtained via numerical calculation. Combined the kinetic description and the thermodynamic phase diagram, membrane formation process were analyzed, which prepared the theoretical foundation for the further membrane formation mechanism research.

论文主要从如下四个方面展开：(1)PVDF疏水膜成膜分相机理探讨基于Flory-Huggins理论推导了三元制膜体系凝胶分相组成的线性表达式，并通过&浊点法&测定了PVDF／N，N-二甲基乙酰胺／水／成膜体系的凝胶边界线组成，通过对实验数据的拟合，得到了简捷直观的线性表达式，ln(φ_1)/(φ_3)=bln(φ_2)/(φ_3)+a，误差小于3％。

Vitro Butea Gel Specific skin care for Men, available in the form of gel for private area application.

化妆品体外凝胶特异性皮肤护理，男性的形式提供，凝胶私人领域的应用。

The paper discusses applications and developments of sol-gel method in the fields of electricity, photics, calorifics and composites, and present problems and development trends of sol-gel chemisty are analyzed in material preparation.

介绍了溶胶凝胶理论和技术的历史背景以及研究现状，评述了近年来在电学、光学、热学以及生物-材料等领域中的应用和发展前景，指出了溶胶凝胶化学在材料制备存在的问题和发展趋势。

Penicillin V acylase from the crude culture filtrate of Fusarium oxysporum FP 941 was purified with a single band on non-denaturing electrophoresis by means of (NH4)2SO4 fractional precipitation, adsorption on, and elution from, celite, ion-exchange column chromatography with DEAE-cellulose, and Sephadex G-200 gel filtration chromatography.

通过硫酸铵沉淀、硅藻土吸附、DEAD-纤维素离子交换层析和Sephadex G-200凝胶过滤，由尖镰孢FP941培养滤液中得到了聚丙烯酰胺凝胶电泳均一的青霉素V酰化酶。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！