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2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点(44、45、49和50号内含子以及5'端启动子区的序列)设计引物,在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾:CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身,经高分辨率的变性聚丙烯酰胺凝电泳后,利用红外激光遗传分析系统及SAGA〓基因分型软件对凝上的扩增产物及内标的位置,进行扫描分析,确定产物片段的大小。

SEM studies of all the three types demonstrate that this colloidal mineral is made up primarily of supermicroscopic low-carbonate fluorapatite aggregates, phosphate microframboids, and radiated fibrous apatite.

对不同矿石类型中磷矿做扫描电镜研究,得知磷矿主要由超微晶低碳氟磷灰石集合体、磷酸盐显微莓群以及纤维放射状磷灰石等构成。

The results show that the cost of the highly effective modified AKD emulsion in neutral sizing is lower than that of the common AKD emulsion, the indices of the paper can reach A-grade standard of fluting base paper and the cur- ing time can be remarkably shortened.

结果表明:采用高效改性AKD乳液进行的中性施,施成本低于普通AKD乳液,成纸的各项指标均能达到A级瓦楞原纸的标准,而熟化时间能明显缩短。

The company has photography, electric points, platemaking, design, drawings, sketch-like, do Prepress superior protection with the world's most advanced computer alcohol Heidelberg, Germany, four multi-color printing presses, a six GTO with a number of printing presses and more Taiwan, a plastic machine, polishing, UV machine, riding nail machine, plastic Machine, Foldout machine, Blocking machine, beer machine, mounted machine Hang complete set of equipment.

公司拥有摄影、电分、制版、设计、绘图、打稿做样等优越的印前保障、拥有世界上最先进的德国海德堡电脑酒精四色印刷机多台、GTO大六开带号码印刷机多台、过机、磨光机、UV机、骑马钉机、装机、折页机、烫金机、啤机、裱坑机等完整的配套设备。

I plant has a team of experienced professional and technical personnel, manipulation of high-speed automatic packing into a sexual machine, automatic fold-machine, large-scale high-precision four-post punch, punch the ordinary type, fully automatic soft-line plastic box machine, fully automatic viscosity All machines, hydraulic and vacuum mixer machine, punching machine, pulling machine, plastic cutting machine, printer point, computer numerical control cutting machine and other advanced production equipment and testing instruments, and is equipped with emergency generators and more ensure delivery.

我工厂拥有一批经验丰富的专业技术人才,操控着高速自动吸塑成性机,全自动折边机,大型高精密四柱冲床,普通型冲床,全自动柔线盒机,全自动粘合机,油压机及真空密炼机,冲片机,拉片机,切机,点印机,电脑数控切条机等先进的生产设备和检测仪器,并配有后备发电机,更能确保交货期。

It happens in glue to make a part commonly (be like large base, medium bottom, foxing, Baotou) with the side the interface between the component is in, also appear in glue to make a part sometimes between, be like a bottom, with come away.

它一般发生在制部件(如大底、中底、围条、包头)和帮部件之间的界面处,有时也出现在制部件之间,如底、跟脱开。

From probability consideration, the curing theory of A〓+A〓-type and A〓+ B〓-type polymerization involving initiation stepis proposed. The sol-gel distribution equations which can describethe behaviour of the conversions in the curing process are givenand the gelation condition, the number fractional distribution andits invariant property are obtained.

针对含引发机制的〓型和〓型缩聚反应体系的固化问题,利用概率分析的方法进行了系统的理论研究,给出了这两类反应体系的溶分配方程,从而揭示了体系在固化过程中的变化规律,得到了这两类反应体系的凝化条件、数量分布函数及其不变性。

The effect of initial pH, temperature and N263 concentratio n on extraction of fucoidan was investigated. It has shown that temperature and initial pH of the solutions have no great influences on the extraction of fucoid an, and when N263 concentration is low, an increase in the concentration incre ases the extraction of fucoidan.

对影响萃取褐藻糖的水溶液初始pH、温度及N263浓度等因素研究结果表明,N263浓度是影响褐藻糖萃取的主要因素,而初始pH、温度等的影响不大。

Expression of islet-1 in spinal cord of adult rat.In grey of the spinal cord of adult rat, the results of both of ISL1 immunohistochemistry and islet-1 mRNA in situ hybridi-zation showed that islet-1 gene expressed predominantly in the nuclei of motoneurons in ventral region and some cell around central canal and dorsal region. In white of spinal cord, the expression of islet-1 located in astroglia in anterior and dorsal funiculus.

在正常成年大鼠脊髓灰质中,ISL-l免疫组化和islet-lmRNA原位分子杂交结果都显示islet-1在腹侧角运动神经元中有相当强度的表达;在中央管周围和背侧角状质也有islet-1阳性表达细胞存在;在脊髓白质中,islet-l在腹侧索和背侧索中的星形质细胞有表达,外侧索相对较少。

The experimental results showed that pinocembrin, chrysin and galangin were the main flavonoids in Chinese propolis.

实验结果表明:乔松素、白杨素和高良姜素是中国蜂的主要黄酮类化合物;我国不同产地的蜂主要化学成分基本相同。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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