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Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上,开展了如下未见文献报道的创新性的研究工作:(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究,建立了微乳电动色谱-激光诱导荧光检测法和束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法,与以前的报道相比,灵敏度和分析时间均有很大改善;(2)使用荧光素钠作为背景荧光试剂,建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法,扩大了激光诱导荧光检测的应用范围,对难衍生化合物的分离分析提供了一种新思路和新途径;(3)以荧光素钠作为背景荧光试剂,建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的束电动色谱—间接激光诱导荧光检测新方法;(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物,为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Euglobulin precipitation combined with gel filtration is a good method for purification IgM Mabs from ascites.

本文通过优球蛋白沉淀结合凝过滤法纯化腹水IgM型抗体,并与传统的硫酸铵沉淀结合凝过滤法相比较。

Thus, the energy hybridization model of silver particles was deduced.(2) Laser ablated silver particles were prepared to characterize chemiadsorption of nucleophilic anion IO_3~-and its evocable spectroscopy.

2利用激光烧蚀法制备了"化学纯"银,研究了碘酸根离子对银表面能级的影响及由此引起的光谱学信号的变化。

DATA SYNTHESIS: Il-17 exerted the biological function after combines with its receptor maybe induced the occurrence and the development of the osteoarthritis by the way of increasing the chondrocyte, expression of inducible nitricoxide synthase and nitrogen monoxide, boosting up the tbe chondrocyte and the expression of MMP-1/3/13, boosting up the activation of the aggrecanase and the collagenase, boosting the decompose of the cartilage proteoglycan and collagen, boosting up the monocyte evocable breakage of the cartilaginous matrix.

资料综合:白细胞介素17通过与其受体结合发挥生物学作用,其可能通过增加关节软骨细胞诱导性一氧化氮合酶表达和一氧化氮产量,增强软骨细胞基质金属蛋白酶(MMP-1/3/13)的表达,增强蛋白聚糖酶和原酶活性,促进软骨蛋白多糖及原降解,并增强单核细胞对软骨基质的破坏,从而诱导和加速骨关节炎的发生和发展。

The tiscosity of the gelatinize layer on the service life of doubleside, the tiscosity gelatinize layer is low, and the second time, plates are not easily glued; sticktness exorbitance rrepublic change version of destructive doubleside surface.

层的黏度差双面的搁置寿命感化较不小,涂层黏度矮,第二次搁置时,版材不易粘牢;黏度功矮。换版时便当爱护双面暗地。

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞(1×105·mL-1)三维原凝表面添加提纯的不同浓度绿脓杆菌外毒素A(0.1、1.0和10 mg·L-1)后,放入无血清MEM培养液中37℃培养24 h,采用光学显微镜观察培养的兔角膜基质细胞形态学变化,采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

Proper-sized coil may reduce expectoration and other complications, and NBCA is more effective than albomin gel to make collapse.

适当大小的螺圈(直径3mm)可减少咳出/移位率和并发症;此外,NBCA 比生物蛋白能更有效地造成肺萎陷。

METHODS: Human umbilical cords were processes within 6 hours with explantation technique, collagenase digestion and enzymes combination digestion after harvested, and followed by counted colony numbers. A colony contains more than 50 cells.

脐带采集后在6 h内分别用植块法、原酶消化法、原酶与胰酶联合消化法进行分离培养,于培养第14天计数集落数,超过50个细胞计为集落。

The structure of Xe, evidenced by TEM and selected-area electron diffractionexperiments, was face centered cubic in an fcc matrix.

而经重辐照(包括γ射线,电子束及重粒子等)的碱卤晶体就会形成碱金属心,这种碱金属心有着与其所在碱卤晶体中碱金属离子相同的立方面心结构,而且其晶格参数与它所在的碱卤晶体相比只有很小的偏差。

It mainly includes mill roll stan,single facer,conveyor bridge,glue machine,double facer,slitter,cut-off,stacker,and steamheating system and glue preparing and supplying system.

主要由纸架、单面瓦楞机、输送过桥、上机、粘合烘干机、分纸压线机、横切机、堆码机以及加热系统、制系统等主要设备组成。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

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