胰酶

Dissolve the pancreatic digest of gelatin, the dibasic potassium phosphate, and the agar in the water, with warming, and allow to cool.

将白明胶胰酶消化物、磷酸氢二钾、琼脂溶解于水中，伴以加温，并静置放凉。

To determine the true rate of aneuploidy and to elucidate the mechanism of aneuploidy,the sperms were decondensed for FISH analysis. It was found that the disomic frequencies of chromosome X was 0.208%. A new decondensation method was developed by trypsin treatment. The basic morphology of sperm was maintained.

为了解非整倍体的真实发生率及其发生机制，本文以X染色体α卫星DNA探针进一步检测出正常精子中X染色体的二体型频率为0.208％，同时，建立了一种保持精子基本形态的胰酶去凝聚的方法，为目前国内尚未开展的精子遗传物质结构的分子细胞遗传学研究，提供了新的手段。

and Result:(1 ) Endogastric administration of tan-extracted Rhubarb had no effecton gastric emptying and pancreatic enzyme secretion,buthepatic-bile secretion was increased.

胃饲去鞣质大黄水浸煎剂和腹腔、静脉给予没食子酸，观察对大鼠体重、摄食量、胃排空速度以及胰酶、肝胆汁分泌量的影响。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料，通过9～11 日龄非免疫鸡胚进行了病毒的实验室分离和传代，并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定，结果表明，4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞，标准阳性血清可特异性的抑制其凝集性，回归试验可复制出与自然发病相同的病例，病毒传代物有明显的致鸡胚矮小化作用，透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子，将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

Cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

Result Cells growed well in DMEM that contain 15% fetal cattle serum, and inoculative density was 4×10^4cellIml, penicillin and streptomycin concentration was 0.05%, pH was 7.2. It was easy to control and have good digest effect that use Hanks washes the cells three times advance then apply 0.05% trypsin-EDTA-Na2H2O to digest for one minute when convergence degree reaches 95-100%.

结果 含15%胎牛血清的DMEM培养基，接种密度为4×10^4个/mL，双抗浓度为0.5%，pH为7.2；在汇合度达95~100%时，Hanks洗3次，0.05%胰酶－EDTA-Na2H2O消化1min，传代效果最好，条件易于控制，且细胞能顺利生长。

A separation method of rat luteal cells was established.

采用胰酶消化法建立了大鼠黄体细胞体外分离方法。

NO.1 chromosome was metacentric, while that of quail was submetacentric.

采用外周血淋巴细胞培养法和胰酶处理法，对家鸡和鹌鹑染色体的核型和G带进行了研究。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。