胰酶

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC，5％CO。培养箱中培养1－2小时后，弃培养液：lxPBS清洗数次；0.25％胰酶－EOTA 37℃消化5分钟，加入含川％FBS的a－MEM，经小25tim过滤网过滤；IX PBS清洗末过滤细胞，即得到纯化的破骨样细胞，并进行细胞形态学和分于生物学鉴定。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

The inhibition of the secretion and release of pancreatic enzymes might be one of the mechanisms responsible for the action.

抑制胰酶的分泌和释放可能是它的作用机制之一。

Isolation purification of NRCs:The NRCs were collected from the heart of neonatal rats,they were digested with steapsin and different speed of adhesive-screeing .

NRCs的分离、纯化：取出生1天的SD乳鼠，用胰酶消化和差速贴壁方法分离、纯化NRCs。

Our factory was founded in 1993, mainly the production of industrial trypsin, in order to soften the leather.

我厂成立于1993年，主要生产工业胰酶，为皮革的软化。

Methods: Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture.

采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。

The plasmid of pEGFP-N1 was preserved in our laboratory.②The rat vibrissa follicles were dissected under a stereomicroscope. The dermal sheath was moved by incubated in dispase. The bulge regions of the hair follicles in anagen phase were carefully cut between the arrector pili muscle and the sebaceous gland, and then incubated with a mixture of trypsin and EDTA. The cell suspension was selected, and cultured in 10% fetal bovine serum DMEM/F12 FAD medium. After 7 days culture, HFSC was obtained by rapid adhering on collagen Ⅳ.

实验方法：大鼠在体视显微镜下分离出含真皮鞘的完整毛囊，dispase消化，将毛囊从真皮鞘中挤出，收集形态完好且处于生长期的毛囊，分别在毛球部上端、皮脂腺下端横切毛囊，取中间部分置于胰酶和EDTA中联合消化，向所得细胞悬液中添加含10%胎牛血清DMEM/F12完全FAD培养基，常规培养7 d后，采用IV型胶原快速贴壁法两次筛选以分离纯化大鼠毛囊干细胞。

Digestive the well-grown cells with pancreatic enzyme(0.25%), count under the microscope, and make them into one-celled suspension, with a density of 1×105/ml.

将培养瓶中生长状况良好处于对数生长期的前脂肪细胞用0.25%的胰酶消化，显微镜下计数并制成密度为1×105个/ml的单细胞悬液备用。

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化，由于V4株属无毒株，其在CEF细胞中很难形成可见的蚀斑，因此我们采用了用胰酶处理V4病毒，并在上层胶中添加尿囊液及Mg2+，这样可以偶尔产生可见的蚀斑，但实验结果重复性很差。

Retrieving capsulated cells for cell counting, MTT assay,CCK-8 assay daily for 8days to determine cell activity,and analysis growth curve.

囊管内细胞胰酶消化回收，以细胞计数、MTT法、CCK-8法测定细胞的活性，并进行生长曲线分析。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。