胰酶

The results showed that:(1) The relative weight gain, special growth rate, feed conversion rate, feed protein efficiency and protein deposition rate were improved significantly (P.05) by supplementing compound acidifier in common carp diet.(2) The protease activities in the liver pancreas, fore and middle intestines increased significantly (P.05) by supplementing 0.3% compound acidifier. The activities of amylase and lipase were improved after the supplementation, but not at significant level.(3) Supplementation of compound acidifier in common carp diet did not significantly affect the fish body protein, lipid, ash, moisture contents, coefficient of fatness and viscerosomatic index.

结果表明：1添加复合酸化剂显著(P.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标；2添加质量分数0.3%的复合酸化剂时，鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P.05)，酸化剂组的淀粉酶和脂肪酶活性有升高的趋势，但与对照组差异不显著；3鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。

First, the CVFF force field was used to build the three dimensional structures of hydrophilization-modified trypsin andα-chymotrypsin based on the crystal structures of native trypsin and αchymotrypsin.

首先，从天然胰蛋白酶和α-胰乳蛋白酶的晶体结构出发，运用CVFF力场，对修饰前后酶分子空间结构进行合理建模。

The results showed that the total numbers of integral membrane proteins identified based on PAOT,trypsin/chymotrypsin and methanol-modified digestions were 101,59 and 72,which included 67,40 and 31 proteins with GRAVY＞0,respectively.

结果表明，甲醇酶解法鉴定到72个整合膜蛋白，其中平均疏水值大于0的蛋白数为31；胰蛋白酶/胰凝乳蛋白酶酶解法共鉴定到59个整合膜蛋白，其中平均疏水值大于0的蛋白数为40个；PAOT法共鉴定到101个整合膜蛋白，其中平均疏水值大于0的蛋白数为67个。

In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分，我们按照交替氧化酶(Alternative Oxidase，AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列，用固相法合成由12个氨基酸残基组成的多肽，将此多肽与α-牛胰凝乳蛋白酶原A相连，用作半抗原，免疫家兔制备人工合成12肽的抗体。

The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基，分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后，分别采用MALDI-TOF-MS分析，其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段，质量数在800以上的NSPC-2有11段，即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。

Fat tissue is hydrolyzed and released fatty acid as well triolein by lipase; on the other hand, high fat diet can't be directly absorbed across the intestinal mucosa.it must be firstly decomposed into one 2-monoglyceride and two free fatty acids by pancreatic lipase ,which is delivered into the lumen of the gut as a constituent of pancreatic juice.

脂肪酶能够使体内的多余脂肪组织分解成三酸甘油酯，另一方面，人体摄入高脂肪食物，并不能直接被小肠粘膜吸收，而是先被小肠内的胰脂肪酶分解成2-单酸甘油酯和两个游离脂肪酸，然后和胰液一起被运输到肠道的内腔被吸收。

Tyrosine and tryptone at the concentration of 4g/L improved the enzyme production, reaching 2.49 and 2.0IU/mL resp...

4g/L的酪氨酸和胰蛋白胨有利于酶的产生，分别可使酶活力最高增加到2 49IU/mL和 2 0IU/mL。

The optimal chitinase fermentation conditions of strain SWCH-6 were conformed by single-factor experiments and orthogonal experiments, they were colloidal chitin 25.0 g/L, tryptone 10.0 g/L, seawater 1 L, pH 8.5, 32℃, 150 r/min for 72 h.

采用单因素优化方法结合正交实验，得到菌株SWCH-6产几丁质酶的最佳发酵条件：胶体几丁质25.0 g/L，胰蛋白胨10.0 g/L，陈海水1.0 L， pH 8.5， 32℃， 150 r/min培养72 h；在该条件下酶活力达0.39 U/mL。

Comparison on the effectiveness of different cryoprotectants with chicken embryo primordial germ cells,Effect of heroin dependence on expression and function of glucagon in islet a cells of rats,Establishment of transgenic mouse line skin-specifically expressing hCTLA4-Ig,Resistance selection by meilingmycin and chlorfenapyr and activity changes of detoxicated enzymes in Tetranychus urticae.

文章摘要：鸡胚胎原始生殖细胞对不同冷冻体系适合性的研究，海洛因依赖对大鼠胰岛A细胞胰高血糖素表达和功能的影响，皮肤组织特异性表达hCTLA4-Ig转基因小鼠品系的建立，二斑叶螨对梅岭霉素和溴虫腈的抗性选育及其解毒酶活力变化，北京市褐家鼠种群遗传多态性及与汉坦病毒分布关系的研究，金乌贼同工酶分析，Nogo—A在成年大鼠脑内神经元的分布。

It is a very basic, single-chain protein with molecular weight about 14 kD. It shares 33% sequence identity with bovine pancreatic RNaseA and has structurally equivalent counterparts for the two histidines and one lysine that comprise the catalytic residues for ribonucleolytic activity.

它是由123个氨基酸组成的分子量约为14kD的单链碱性蛋白质，与牛胰核糖核酸酶有33％的同源性，两个组氨酸和一个赖氨酸残基组成了血管生成素核糖核酸酶活性中心。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。