胰酶

The inactive precursor of trypsin, produced by the pancreas and converted to trypsin in the small intestine by enterokinase.

胰蛋白酶原胰朊酶的惰性前体；由胰制造并由肠激酶在小肠内转化为胰朊酶

In the small intestine by enterokinase.

胰蛋白酶原胰朊酶的惰性前体；由胰制造并由肠激酶在小肠内转化为胰朊酶

We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时，我们以人类CNE1细胞系为材料，比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法，发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍，并且这些整合膜蛋白的疏水值大部分都为正值。

RESULTS The activity of the pancreatin reached 3.70×10^3, 2.80×10^4 and 5.30×10^4Ug^(-1) respectively as 6-8 times as the requirement in ChP standard. Recovers yield of the method was above 15%.

结果 收率达15%以上，胰蛋白酶、胰脂肪酶、胰淀粉胰三酶活力分别为3.70×10^3、2.80×10^4、5.30×10^4Ug^(-1)，为《中国药典》要求的6~8倍。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P＜0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P＜0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P＞0.05). Enzymepreparation significantly increased (P＜0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P＞0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二：酶制剂显著提高平均日增重和饲料转化率(P＜0.05)；酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P＜0.05)，而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著；酶制剂显著降低十二指肠和空肠食糜相对粘度(P＜0.05)；添加酶制剂对肠道pH影响不显著；酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P＜0.05)，显著提高十二指肠和空肠绒毛高度，显著增加十二指肠和回肠绒毛高度／隐窝深度，降低回肠隐窝深度(P＜0.05)，对肠壁厚度影响不显著；酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P＜0.05)，对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著，显著提高胰岛素、T_3、IGF-Ⅰ水平，添加酶制剂后，促甲状腺激素、生长激素分别提高16.44％、19.18％和18.84％、21.74％，胰高血糖素和T_4分别降低12.07％、14.36％和13.79％、15.40％，但差异不显著；酶制剂对胰腺消化酶活性影响不显著，显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性，对小肠脂肪酶活性影响不显著；酶制剂对盲肠微生物菌落数影响不显著。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶，分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后，再加入脂解酶反应液中观察活性的变化，结果发现脂解酶的活性未如预期，没有抑制的效果产生，将甘薯beta-淀粉酶以同样方法与胰脂解酶作用，结果同上述两种beta淀粉酶依旧没有抑制的现象。

The activities of amylase from high to low were pancreas, the midgut, the hindgut, the foregut, the stomach and the esophagus, respectively.

脂肪酶活力大小顺序依次为胰脏＞后肠＞中肠＞前肠＞胃＞食道，胃、肠和胰脏脂肪酶的最适pH值分别为2.2、7.4和8.0，最适温度均为50℃。

While , the amylase and lipase activities in stomach were significantly higher than those in intestine and hepatopancreas. In C. asiatica adults, the protease activity was highest in midgut mucosa with lowest in stomach mucosa. The trypsin activity was highest in midgut mucosa and lowest in hindgut mucosa. While, the amylase activity was highest in hindgut mucosa with lowest in foregut mucosa, and the lipase activity in the stomach mucosa was distinctly higher than that in other digestive organ or tissues.

月鳢成鱼蛋白酶活性以中肠粘膜组织和后肠粘膜组织最高，前肠粘膜和肝胰脏次之，胃粘膜最低；胰蛋白酶活性以中肠粘膜组织和前肠粘膜最高，胃粘膜组织次之，肝胰脏小于胃粘膜，后肠粘膜组织的胰蛋白酶活性最小；淀粉酶活性以后肠粘膜组织和中肠粘膜组织最大，肝胰脏次之，胃粘膜组织和前肠粘膜组织最小；脂肪酶活性以胃粘膜组织最大，其他器官组织该酶活性均表现较小。

Methods The retinae of 6 postnatal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion. The cell suspension was implanted in 96-well culture plates covered with murine-tail collagen in each well(1×105 cells/well). The concentrations of PGP solution were 1g.L-1-10-5g.L-1. The period of culture was 1,3 and 5 days respectively, Then, the OD of living cells in each well was tested by MTT colorimetric microassay.

6只生后2-3d Sprague-Dawley大鼠，视网膜采用胰酶消化法制成细胞悬液后接种于经鼠尾胶原处理的96孔培养板(每孔1×105个细胞)，同时加入1g.L-1-10-5g.L-1PGP及DMEM，分别培养1、3、5d，用MTT法测量OD值。

2 BJP direct toxicity on TEC NRK52E cell were subcultured on a 24-well plate (3×10〓/ml/well) and exposed to BJP κ or BJP λ in concentration 1, 5, 10, 20mg/ml for up to 24h, then the media was changed, and 〓H-thymidine was added for incubation for 18h at 37℃, the corporation was terminated, Acid-insoluble DNA was precipitated by adding 5% trichloroacetic acid radioactivity in the extracts was determined in scintillation counter.

细胞增殖的影响：将TEC株NRK52E以3×10〓/ml/孔分别加入24孔培养板，分别加入BJPκ与BJPλ（浓度1、5、10、20mg/ml）共同培养，均设3个复孔，24小时后加入〓H-TdR（1μCi/孔）在5%CO〓37℃孵育18小时，终止反应，加胰酶脱壁半小时后移到普通干燥试管，多头细胞收集器抽滤，用5%三氯醋酸，无水乙醇分别冲洗，烤干，冷却后加入闪烁液，放入液体闪烁计数仪测定放射性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。