胰蛋白酶原

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

Methods The Epidermal stem cell were isolated by adhering to collagen type IV after obtained by digesting human foreskin with Dispase Ⅱ and Tryp and culture in vitro in K-SFM. The expressions of β1-integrin and keratin 19 (K19) in epidermal stem cell were detected with immunocytochemical methods,and the colony forming efficiency was also studied .

中性蛋白酶和胰蛋白酶两步法从手术切除的人包皮组织中分离表皮层和真皮层，并获得表皮单细胞悬液，采用Ⅳ型胶原铺板选择性粘附、分离和角质形成细胞无血清培养基培养表皮干细胞。

Objective: Mast cell tryptase has been implicated in regulation of skin fibroblast, lung fibroblast and hepatic stellate cell proliferation and collagen synthesis.

目的：肥大细胞类胰蛋白酶已被证明在皮肤、肺成纤维细胞和肝星型细胞增生和胶原合成中具有重要作用。

T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

METHODS: The skeletal muscle samples were obtained from 20 to 25-week abortion fetus, the family history of inherited myopathies of parental generation was negative.

选择健康妇女捐赠的胎儿骨骼肌标本，参照 Blau等的胰蛋白酶与胶原酶混合、多步消化法分离成肌细胞。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Using an anticoagulant it was possible to isolate plasma and intact haemocytes from Helicoverpa armigera larvae. PO and ProPO activities in plasma and haemocyte lysate were assayed when using bovine trypsin as an activator and L-DOPA as a substrate.

用抗凝剂从棉铃虫血淋巴中分离获得了血浆及完整的血细胞，以L-DOPA为底物，牛胰蛋白酶为激活剂，测定了血浆及血细胞裂解液中酚氧化酶及酚氧化酶原的活性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。