胰蛋白酶原

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment. The bone pieces were digested by 0.25% trypsin for 30 min at 37℃ at first, then digested by 0.2% collagenase at 37℃ in constant temperature oscillator for 60 min, and rinsed repeatedly to disaggregate more single-cells.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

However allergen,lectins and chymotrypsin inhibitor impacts on Health and Food Safety.

但花生也有一些缺点，花生中含有胰蛋白酶抑制剂、过敏原、凝集素等抗营养成份，影响健康和食品安全。

The pancreatic proterses trypsin,chymotrypsin and elastase are all derived from zymogen precursors by proteolytic activation.

胰腺的蛋白水解酶—胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶都从酶原前体衍生而来，即由蛋白水解而活化的。

Methods PMCs were isolated from rat colic omentum and the 3rd generation cells were used in the experiment.

胰蛋白酶消化法用于PMC的原代培养和传代，经鉴定，第3代细胞用于实验。

Methods:PAEC was isolated by collagenase and pancreatin digestion and was put into 0.5×104 cells per well.

胶原酶和胰蛋白酶消化猪主动脉内膜，制备3～6代血管内皮细胞的单细胞悬液。

Type Ⅰ collagenase and trypsin were used for this study.

型胶原酶为美国Gibco公司产品，胰蛋白酶为美国Sigma公司产品。

Context All work on human trypsinogen actiation peptide in acute pancreatitis has been carried out with the same assay.

背景：目前几乎所有的人急性胰腺炎胰蛋白酶激活肽的检测仅有一种化验方法，即检测原TPA含量。

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

The deletion of [A7-B7] disulfide bond in proinsulin resulted in a significant decrease of α-helix content of the molecule and a great increase in sensitivity to tryptic digestion.

结果表明，A7 B7二硫键缺失可导致胰岛素原α螺旋明显减少以及对胰蛋白酶的酶切敏感性显著增加，其对胰岛素原结构的影响主要导致了受体结合活性的大幅度降低。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。