胞质网

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

As a member of septation initiation network, sepH is a key factor of cytokinesis in Aspergillus nidulans. However, how the suppressors of sepH affect cytokinesis is not yet understood.

在构巢曲霉Aspergillus nidulans的细胞隔膜开始网（septation initiation network，SIN）的信号途径中，sepH基因对胞质分裂起着关键的正调节作用，但对该途径中同样起着十分重要作用的有关反向调节子的研究目前还不清楚。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC，再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域，则CTC膜结合Ca〓呈现弥散的荧光影像，在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认，说明除了线粒体之外，还有其它含Ca〓细胞器，也被CTC染色，线粒体并不是细胞内唯一Ca〓库，还可能包括内质网、高尔基体等细胞器，但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色，最大荧光强度仍位于菌丝顶端2μm以后区域，约45μm以后荧光变弱，实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

Results By using zinc-iodide-osmium staining, the tubulo-vesicular system of parietal cells of fundic gland was manifested as numerous fibrous structures under HVEM. The nematolysosomes was often demonstrated as branching in shape and anastomosed with each other making a network system in cytoplasm under the visual field of the three dimensional electron microscopy combined with AcPase cytochemistry.

结果高压电镜下显示，锌-碘-锇酸染色方法制备的标本胃底腺壁细胞的微管泡系统呈无数纤维状结构；AcPase结合三维电子显微镜观察显示，线性线粒体在胞质中常呈分支状并互相吻合成网。

Stimulated astrocytes with Acetyl-β-Methylchline Chloride , Caffeine , Thapsigargin and carbonyl cyanide p- trifluomethoxphenylhydrazone by different types of combination, and observed their effects on 〓.

发现星型胶质细胞内的钙库包括：〓敏感和non—〓敏感的两种内质网钙库，可分别被MCh和CAF激活；线粒体钙库参与胞浆内Ca2+缓冲系统。

As a sequence, the cell evolved an adaptively coordinated response to limit further accumulation of unfolded proteins by expressing large amount of chaperons. In this pathway, Ite1, an ER transmembrane protein kinase/endoribonuclease, is essential for viability during ER stress.

当内质网腔内未折叠蛋白增多时，通过Irelp胞质面部分的丝氨酸／苏氨酸自磷酸化，诱导UPR特异性转录因子Haclp mRNA剪接成熟。

Transmission electron microscopy demonstrated that fibroblast was full of endoplasmic reticulums that cisternal-like expanded in the rhEGF nanoparticle group, and collagen proteins were synthesized in cavity.

透射电镜下可见rhEGF纳米微球组成纤维细胞胞质内有大量的内质网，扩张成池状，腔内充满了合成的胶原蛋白。

Results: Embryoid bodies grew well in Collagen Gel, the differentiated cells grew into the gel gradually; electronmicroscope showed that some induced cells were rich in Golgi's bodies and endoplasmic reticulums, a few secretory granules were also visible, which was similar to human adult thyrocyte.

结果 拟胚体在鼠尾胶原上生长状态良好，分化细胞贴壁后逐渐陷入鼠尾胶原；电镜下诱导细胞高尔基体、内质网丰富，部分诱导细胞胞质内见不典型分泌颗粒，与人成体甲状腺细胞超微结构类似。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。