胞质皱缩

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

Results An adequately cellular cervical cytology smear,the reduce of cells overlay, the integrality of cells morphology, the clear structure of cells and unconspicuous phenomenon of dissolving and cell shrinkage were observed , the cells were stained well and flamboyantly, the contrast ration was outstanding between cytoplasm and nucleu, the cytoplasm, nucleus,karyosome,and other components were easy to be resolved, the cells boundary,transparence showed clearly.

结果：涂片的细胞量充足，重叠减少、形态结构清晰，无皱缩自溶现象，着色良好，色彩艳丽，浆核对比突出，胞质、核染色质、核仁和其他细胞成分易于识别，细胞境界清楚，透明度良好。

N perifocal tissue intracerebral hemorrhage there were rarefaction neuron,cell spaces augmentation,cell diminution,distinct demarcation of cell membrane and surrounding,and we discovered a lot of degeneration and necrosis nerve cells,cell body collapsed,pycnosis anachromasis,nucleoli disappeared. In EPO group we discovered that center area of hemorrhage shinked,nerve cells of degeneration and necrosis decreased in perifocal tissue,majority cells morphous were normal and pathological changes were light.

CH对照组在术后皮层出血中心无神经元，仅见少量胶质细胞，细胞间质空泡样改变；出血边缘区神经元稀疏，细胞间隙增大，细胞缩小，胞膜与周围分界清楚，并可见大量变性及坏死的神经细胞，表现为胞体皱缩，核固缩深染，核仁消失。rhEPO治疗组出血中心区变小，边缘区神经细胞变性坏死减少，多数存活细胞形态相对正常，病变较轻。

Results Lactacystin dealed with C6 glioma cells in 24 h, kytoplasm was concentrated and anachomasised, the chromatin of nucleus was concentrated pyknoticly to see under photo-microscope. The microvilli on surface dispeared、nucleus crenated、chromatin aggregated pyknoticly under transmission electron microscope respectively.

结果 Lactacystin处理体外C6胶质瘤细胞24 h后，光镜下见胞浆浓缩深染，胞核染色质致密浓缩，透射电镜下见细胞表面微绒毛消失，核皱缩，染色质致密浓缩、边集。

Volume of cell ill will color is larger, polygonal, afterbirth film is clear, afterbirth qualitative translucence is fine meshy, afterbirth nucleus shrinkage, visible nucleus channel and nuclear different, nucleolus is unidentified show; and acidity of qualitative be addicted to of afterbirth of acerbity cell of be addicted to submits fine grained form, apparent nuclear week sky is dizzy it is thus clear that.

嫌色细胞体积较大，多角形，胞膜清楚，胞质半透明细网状，胞核皱缩，可见核沟及核异型，核仁不明显；而嗜酸细胞胞质嗜酸性呈细颗粒状，可见明显的核周空晕。

After treatment with AT9810,the cell became smallerand condensed,typical signs,such as cytoplasmicconcentration,chromatin agglutination,pyknotic necleus,necleus cleavage,apoptosis corpuscle,was found inmorphological observation.

经AT9810处理后的细胞体积变小皱缩，形态学观察具有典型凋亡细胞的特征：胞浆浓缩，染色质凝集，核固缩、裂解及凋亡小体形成。

To explain relationship between the effect of CLD on senile Demention and neuron in hippocampal area. The change of natural aging mice's neuron in hippocampal area were studied by applying microscope and transilluminating electric microscope. Results: Comparing with the young control, the old control mice's hippocampus had less small pyramid cells, the nerve cells denaturalizing and their branches reducing in the microscope. Hippocampal nerve cells were degenemiceing, with nucleus membrance crinkling like wave shape were not clear, and chromatin condensing with high electron density, heterochromatin accruing, part cell organs inclosing nucleus, nucleus condensing in the EM.

为了阐明菖龙丹抗老年性痴呆作用与海马区神经元结构的关系，运用光镜和透射电镜观察了菖龙丹对自然衰老小鼠海马区形态的影响，结果显示老年对照组小鼠光镜下海马锥体细胞较青年对照组数量减少，细胞体积变小，神经元变性，分枝减少；电镜下海马神经细胞发生退化改变，核膜皱缩，呈波浪状，膜结构不清晰，核染色质浓缩，电子密度高，异染色质较多，部分胞浆细胞器向细胞核聚集，有的可见细胞核固缩。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降，上述过程因受酸性阻抑而延缓，细胞增殖速度也受影响，处于酸性环境中的软骨细胞呈退行性变化，表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少，且与粗面内质网结合的少，游离在胞浆中的多。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。