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OPN Immuno-electron microscopy show the gold markers in thecraniopharyngioma cell of the ameloblast type be located on the endoplasmicreticulum that the swollen oncocyte afterbirth strach, from endoplasmic reticulumcreation behind secrete the outside of the afterbirth; But didnt see the gold markers inthe craniopharyngioma cell of the squamous type.

OPN免疫电镜显示成釉细胞型颅咽管瘤中金标OPN颗粒的产生位于肿瘤细胞胞浆的内质网上,由内质网产生后分泌到胞外;而在鳞状细胞型颅咽管瘤细胞中未见金标OPN颗粒,少量可见颗粒仅见于肿瘤间质中的血管内皮细胞上。

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示,未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色,核区较暗;CDDP及β-Ele处理组细胞为暗绿色,其中可见皱缩的凋亡细胞;THP处理的细胞胞质荧光弱呈灰绿色,由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和,胞核被染成桔红色,结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

Numbers of secretory granules, oplasmic reticulums, mitochondrias in ACTH cells all changed distinctly. GTH cytoplast were full of big and small, high density electron secretory granules.STH cells were in activity synthesis and secretion, secretory granules were from fusion to release. TSH cells cytoplast evacuated secretory granules, electron density decreased and vacuofes appeared.

ACTH细胞分泌物颗粒数量、内质网及线粒体均发生明显变化;GTH细胞胞质中充满电子密度较高的大、小两种分泌颗粒;STH细胞处于活跃的合成和分泌状态,分泌颗粒呈现出由融合到释放的状态;TSH细胞胞质中分泌颗粒排空,且电子密度降低,出现大空泡。

RESULTS: Compared with control group, the activity of the LDH/ACP/Arg in the AMФ or PMФ and in the culture fluid of the AMФ or PMФ were significantly increased (P.05 or P.01), and the ability devouring neutral red and stimulating index in the PMФ were strengthened (P.01), the ergastoplasm was hyperplasia and the amount of lysosome was manifold in the spleen macrophages of old rats induced by paecilomyces cicadidae total polysaccharides.

结果:蝉拟青霉总多糖使老龄大鼠肺泡巨噬细胞、腹腔巨噬细胞胞内和细胞培养上清液内ACP、LDH、arginase活力显著高于对照组(P.05或P.01);使PMФ的吞噬功能及其刺激指数显著高于对照组(均P.01);使老龄大鼠脾脏细胞胞质粗面内质网和溶酶体数量多于对照组。

Under the light microscope, we found that transfected SGC7901 gastric cancer cells with NHE1 antisense gene were larger with abundant cytoplasm and comparatively same size of nuclei, and the ratio of nuclei to cytoplasm, mitotic count and heteromorphism decreased when they were compared with their parent cells.

与亲本细胞相比,光镜下NHE1反义基因转染的SGC7901胃癌细胞胞体增大,胞质丰富,核大小相对一致,核质比和核分裂象减少,异型性减少。

Results: The main ultrastructural changes after Cd treatment included swelling or vacuolation of mitochondria, increase of myelin figures in GCT cells, and swelling of mitochondria in the endothelial cells of peritubular capillaries. The changes above were most prominent at 7~15d, and swelling of the cytoplasm and slight karyolysis were also found. The morphology of GCT cells didn't recovery to normal yet after 30d.

结果:镉注射后24h,GCT细胞内出现不同程度的线粒体肿胀和空泡化,胞质和线粒体内可见髓样结构,GCT周围毛细血管内皮细胞线粒体肿胀。7~15天后上述改变明显加重,部分GCT细胞见胞质广泛肿胀和轻度核溶解,30天后细胞形态仍未恢复正常。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物,从猪卵巢上获得的卵母细胞数量虽然较多,但成熟度比较低;由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内,跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应;如果卵胞质成熟或活化程度不够,加之圆形未成熟精细胞成熟度比较差等原因,很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足,引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等,使ROSI显微受精失败。

At interphase, there is no cytoplasmic channel in the cell walls of PMCs; At zygonema and pachynema, a lot of cytoplasmic channel, ranging in diameter from 150nm to 600nm, appears; After pachynema, the number of channels decrease rapidly.

在间期的花粉母细胞间未见胞质通道,胞质通道在偶线期前后形成并开放,在粗线期以后迅速减少。在细线期和偶线期,花粉母细胞细胞质膜与细胞壁之间的质外体出现大量的小泡,即所谓的壁旁体。

After the cells dealt with GS-Rh2 of 13.0 mg/L, the typical apoptosis cells were discovered: showing disorganized, highly contracted and vacuolated, condensed cytoplasm, vanished karyotheca, ruptured cell nucleus and apoptotic body.

凋亡细胞吉姆萨染色检测:经13.0 mg/L人参皂甙单体Rh2处理后,HL60细胞出现核染色质浓缩、呈周边凝聚,核膜裂解形成凋亡小体、胞质出现空泡但胞膜完整的典型细胞凋亡形态学改变。

Immunohistochemistry results showed that the gray of PC12 cell cytoplasm appeared shallowing after HFS, suggesting that tyrosine hydroxylase activity decreased; The numbers of visible mitochondria in the cytoplasm, secretory granules, processes and synapse were decreased.

结果:相比对照组,HFS后PC12类神经细胞光镜下突起长度显著缩短,突起个数明显减少;酪氨酸羟化酶免疫组织化学结果显示HFS后PC12细胞胞质灰度降低,提示酪氨酸羟化酶代谢水平下降;电镜下可见胞质中的线粒体、分泌颗粒减少,突起和突触减少。

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