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胞质杂种

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The distribution of hybrid chlorosis Ch1 gene and the T-type cytoplasm fertility restoring genes in Chinese endemic wheats were studied using the testors QA1104 (T-type cytoplasm sterile line, with hybrid chlorosis Ch2 gene) and Khapli emmer (with hybrid chlorosis Ch1 gene).

本研究以T型不育系QA1104(具有杂种黄化基因Ch2)和Khapli(具有杂种黄化基因Ch1)为测验种,对中国特有小麦等六倍体小麦类型和一些四倍体小麦类型中的T型胞质育性恢复基因和杂种黄化Ch1基因的分布进行了研究。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只,无菌抽取犬骨髓液10 mL,经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞,传2代后行5-氮胞苷诱导,5-溴脱氧尿苷标记备用。

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带,且综合双亲的所有带,但在部分杂种中检测到了双亲都没有的新带,也发现有丢失亲本的特征带或共有带的现象,说明融合后染色体发生了重组和交换;有的引物只检测到叶肉亲本的特征带,根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实,可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种

Sour orange and sweet orange may be the hybrid of pummelo and mandarin.

6根据胞质基因组的差异和聚类分析的结果对一些生物型的起源进行了分析,得出如下一些结论:葡萄柚是柚与甜橙的杂种,可能是与柚的回交种;本研究所包括的两个来檬生物型,其中之一的起源与枸橼、柚和澳洲指橘有关,另一个被误认为是枸橼的来檬生物型最后被认定为墨西哥来檬,来源与大翼橙和枸橼有关;粗柠檬是枸橼与宽皮橘的杂种;红黎檬可能是宽皮橘与粗柠檬的杂种;酸橙和甜橙为柚与宽皮橘的后代。

In order to study the effects of NEA male sterile cytoplasm on major characters of F1, the differences between the male sterile and normal cytoplasms of the NEA plants were investigated.

为了探讨NEA细胞质相对效应,对选育出的甘蓝型细胞质雄性不育新材料NEA的不育胞质和可育胞质杂种F112个主要性状进行了比较研究。

The results showed that except pods of main inflorescence, the other characters of the sterile and normal cytoplasms had not significant difference by t test. The correlation coefficients of length of main inflorescence, No. of first time branches and glucosinolates between sterile and normal cytoplasms was at 5 percent significant level, but correlation coefficients of the others was not significant. There were positive effects of male sterile cytoplasm on glucosinolates and weight of 1000 seeds, but different effects on the other characters when combinations were different.

结果表明:考察的12个主要性状中,除主序角果数不育胞质杂种F1和可育胞质杂种F1检验差异达5%显著水平外,其余11个性状2种胞质杂种F1上标 t检验差异不显著;主序有效长、第一次分枝数、硫苷等3个性状的可育胞质与不育胞质间线性相关关系显著,其余9个性状的线性相关关系不显著;NEA不育胞质对多数组合的硫苷和千粒重有优良相对效应,对其余性状的相对效应则组合不同正负效应不同。

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