胞质基因

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

OBJECTIVE: To investigate transfection and expression of liposome-mediated cytosine deaminase gene in rabbit BMSCs.

目的：观察脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其表达。

RESULTS: We cloned cytosine deaminase gene and the results from gene sequencing were the same as Genbank.

将胞嘧啶脱氨酶基因亚克隆到pIRES2-AcGFP1质粒上，构建了pIRES2-AcGFP1-CD真核表达载体。

Given the known reaction mechanism and general chemical considerations, we propose that nicotine, when used as the carbon source by P. putida J5, is catabolized to pyruvic acid via pyrrolidine pathway and then participates in the synthesis of vitamin pantothenate and CoA, which supply the energy for the normal functioning.

通过对突变位点基因的功能分析推测，假单胞杆菌可以将尼古丁代谢为2，5-二羟基吡啶，而2，5-二羟基吡啶可以进一步转化为丁烯二酸和丙酮酸，进而参与了泛酸和 CoA 的合成，为生理代谢提供基质。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Soybean cultivar "Ludou 4" was inoculated with the AM fungus Glomus fasciculatus, soybean cyst nematode Heterodera glycines race 4 and the AM fungus plus SCN. A number of coefficients such as mycorrhizal colonization, SCN infection rate, activities of chitinase and PAL and transcripts of the genes Chibl(Chitinase b1) and PAL5 in soybean roots were assayed.

本研究系统分析了大豆(品种：'鲁豆4')接种AM真菌Glomusfasciculatum和胞囊线虫(SCN，Heterodera glycines)4号生理小种后各处理菌根和线虫侵染率、几丁质酶和苯丙氨酸解氨酶活性及几丁质酶基因Chib1和苯丙氨酸解氨酶基因PAL5转录物的动态变化。

Finally we studied the secretion expression of TGF β1 mature peptide gene in the periplasm and extracellular medium respectively.

在胞内表达TGFβ1基因的同时，还研究了TGFβ1基因在周质和胞外培养基中的分泌表达。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R. leguminosarum chromosomal hurL gene is also involved in controlling its capacity in inducing nodulation on host plant.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研(来源：Acf6fBf6C论文网www.abclunwen.com)究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。