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胞质基因

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Septin was first identified in yeast as a protein that played a role in cytokinesis.

最初认为septin家族是与酵母细胞胞质分裂相关的基因家族。

Over the last 10 years, antisense transgenic plants have been used as tools to address this and have revealed some unexpected findings. In this article, chloroplast tpi gene from spinach and tandem genes of chloroplast tpi gene from spinach and cytoplast fba gene from rice were transferred into filamentous, heterocystour cyanobacterium Anabaena sp.

将菠菜叶绿体tpi和水稻胞质fba的串联基因连接在光诱导的高效启动子PpsbA下游,克隆入pDC-08中构建了既能在大肠杆菌又能在蓝藻中表达的穿梭表达载体pDC-AT,并通过三亲接合转移转入鱼腥藻7120。

Conculsion hTERT RNAi may suppress hTERT expression to result in reduction of Bcl-2 and increase of Bax, and then induce hepatocarcinoma HepG2 cell apoptosis by mitochondrial pathway subsequent to cyt C release from mitochondria to cytoplast.

RNAi靶向抑制hTERT基因可通过抑制Bcl-2表达和促进Bax表达引起线粒体cyt C释入胞质,激活caspase-9促进HepG2肝癌细胞凋亡。

At early stage after brain injury, there were a quantity of fibrous callus and cartilaginous callus formation in brain injury and fracture group and many neuropeptides immunoreactive nerve fibers in callus were found. Strong immunoreactivites of CGRP, SP, VIP, NPY, TOH occurred to osteogenitor cells and chondroblast, which proliferated in thickened endothecium.

脑损伤合并骨折组早期形成大量纤维骨痂和软骨骨痂,骨痂中神经肽免疫阳性神经纤维较多,明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞质内降钙素基因相关肽、P物质、血管活性肠肽、酪氨酸羟化酶、神经肽Y强阳性表达。

In the lung cancer cell line A549, Folliculin is expressed in the cytoplasm and BHD gene is a metastasis suppressor gene.

BHD表达产物Folliculin蛋白在肺癌细胞的胞质内表达,BHD可能是肺癌转移抑制基因。

After the recombinant plasmid pAd-VP linearized with PacI transferred into HEK-293A cell,the recombinant virus was isolated and purified in HEK-293A cells by three times plaque purification.

RT-PCR 检测证明目的基因在 mRNA 水平上可有效表达;应用 O 型口蹄疫病毒标准阳性血清进行间接荧光抗体试验,在 rAd-VP 感染的 HEK-293A 细胞的胞质可见清晰荧光。

Then, the experiment that edelfosine inhibited the phosphatization of Spm1 was carried out in order to elucidate whether edelfosine affect on the phosphatization of Spm1 induced via Mid2 and inhibited via Pmp1.(1)、On the inhibition experiment of parallel growth of S. pombe wild-type cells, spm1 mutants, pmp1 mutants, mid2 mutants and relevant retransform strains, the spm1 mutants grew well treated with 5.0μM and 10.0μM edelfosine for 24 h; the growth ratio of spm1 mutants had a statistic significance in between the cells previously mentioned and the S.

(1)、通过野生型粟酒裂殖酵母和胞质分裂突变体spm1Δ、pmp1Δ、mid2Δ及其再转化株对依地福新的平行生长抵抗试验,进一步确定依地福新对spm1、pmp1、mid2基因的影响;(2)、应用依地福新对Spm1磷酸化的影响试验,阐明依地福新是否通过影响Mid2蛋白和Pmp1蛋白的表达而影响Spm1蛋白的磷酸化。

As a member of septation initiation network, sepH is a key factor of cytokinesis in Aspergillus nidulans. However, how the suppressors of sepH affect cytokinesis is not yet understood.

在构巢曲霉Aspergillus nidulans的细胞隔膜开始网(septation initiation network,SIN)的信号途径中,sepH基因对胞质分裂起着关键的正调节作用,但对该途径中同样起着十分重要作用的有关反向调节子的研究目前还不清楚。

Methods Lymphocytes with different MTHFR C677T genotypes from the donors were cultured for 9 days in media with different concentrations of folic acid and the frequencies of micronucleated binucleated cell was evaluated by cytokinesisblock micronucleus cytome assay.

采用9天淋巴细胞长期培养辅以胞质阻断微核细胞组分析,分析不同浓度的叶酸对三种MTHFR C677T基因型乳腺癌人群和健康人群双核细胞微核率的影响与差异。

This study focused on the mRNA decay process of the transcripts of the candidate sterile gene in maize Zea mays L.

本研究对玉米S型细胞质雄性不育中胞质不育候选基因的转录产物在核恢复基因的作用下被降解并引起育性恢复的生物学机理进行了探讨。

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