胞质基因

The effect of the specific inhibitor cibacron blue of GSTs and antiparasitic drugs albendazole, praziquantel and artesunate on the activities of recombinant GSTM, GST1 and GST2 was evaluated.

研究目的解析三个胞质GSTs的基因结构及其特点，克隆三个胞质GSTs基因，鉴定原核表达的重组GSTs的免疫反应性、免疫原性、催化活性及其免疫定位和抑制剂、常用抗寄生虫药对催化活性的影响。

The distribution of hybrid chlorosis Ch1 gene and the T-type cytoplasm fertility restoring genes in Chinese endemic wheats were studied using the testors QA1104 (T-type cytoplasm sterile line, with hybrid chlorosis Ch2 gene) and Khapli emmer (with hybrid chlorosis Ch1 gene).

本研究以T型不育系QA1104（具有杂种黄化基因Ch2）和Khapli（具有杂种黄化基因Ch1）为测验种，对中国特有小麦等六倍体小麦类型和一些四倍体小麦类型中的T型胞质育性恢复基因和杂种黄化Ch1基因的分布进行了研究。

Under the light microscope, we found that transfected SGC7901 gastric cancer cells with NHE1 antisense gene were larger with abundant cytoplasm and comparatively same size of nuclei, and the ratio of nuclei to cytoplasm, mitotic count and heteromorphism decreased when they were compared with their parent cells.

与亲本细胞相比，光镜下NHE1反义基因转染的SGC7901胃癌细胞胞体增大，胞质丰富，核大小相对一致，核质比和核分裂象减少，异型性减少。

Taking self-fruitful rate as the criterion and segregating population of field survey as method, we got the result that the ratio of fertile plants and sterile plants in the three F2 population of K-cytoplasmic male sterile hybrid accords with 63:1 tested by Chi-square, therefore we can infer that the restorative mechanism of K-cytoplasmic male sterile line was controlled by three different major genes, which manifested as sporophore sterility.

结果表明：在三个K型胞质雄性不育的F2群体中，可育单株与不育单株的比例经卡方检验符合63：1的理论比例，因此可以推断小麦K型胞质雄性不育的恢复由3对主效恢复基因控制，并表现为孢子体不育类型。

Rbkinesin-6 plays an important role in telophase/cytokinesis phase, Rbkinesin-6 ablation can inhibit the growth of lung cancer cells.

Rbkinesin-6在A549细胞有丝分裂末期／胞质分裂最后阶段起重要作用，Rbkinesin-6基因沉默可以抑制肺癌细胞的生长，为肺癌基因治疗的研究提供了新的思路。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法，以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白，获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid／lymphoid or mixed-lineage leukemia 3)等几个候选蛋白，其中NAC1、MLL3与基因的转录调控有关，DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关，而BicD2与高尔基体的调控紧密相关，提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

It is suggested that the algae symbiot can affect host organellar function,quantity and location, stimulate the food uptake and digestive activity, restrain the functional activity of the organelle, change the gene expression and cause the change of genome structiure in the P. bursaria-chlorellae system.

据结果推测，纤毛虫中的藻类共生体可影响宿主细胞中胞器的功能、数量和分布，可影响细胞核核仁的数量和分布；可影响宿主细胞的形态、生长、抗氧化能力及胞质糖含量；可影响宿主细胞的食物摄取及相关胞器的消化活性，抑制宿主胞器的功能活动；并可改变宿主细胞基因的表达，导致细胞基因组结构的变化。

Cytoplasmic type, tissuey type and nuclear background had little influence on RNA editing of the two genes, but each factor had its own influence tendency on plant editing frequency of the same or different genes.

胞质类型、组织类型和核背景对两个基因的RNA编辑位点并没有太大影响，但是各因素对植物相同或不同基因的编辑频率表现出各自不同的影响趋势。

After lock out of the 5LO gene by homologous recombination in embryonic stem cells, 5LO deficient-mice can develop normally and stay healthy, with a selective opposition to certain inflammatory insults. The cellular distribution of 5LO in unstimulated cells among different cell types is different; it can present in the cytosol, the nucleus, or both.

采用同源重组方式敲除胚胎干细胞中的5-脂氧合酶基因，小鼠能正常生长和发育，但对某些炎症损伤的反应性与正常小鼠不同。5-脂氧合酶在不同的细胞中表现出不同的亚细胞定位，分布于胞质或胞核，或两者都有。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。