胞质体

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

OBJECTIVE: To investigate transfection and expression of liposome-mediated cytosine deaminase gene in rabbit BMSCs.

目的：观察脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其表达。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

The CNE2/Gem cells appeared roundish in various size under light microscopy, with more granulation in cytoplasm.

光镜下见CNE2/Gem胞体变圆，大小不一，胞质内有较多颗粒。

The main target of PDT-CPD4 was probably membrance organelles, such as cytomembrance、lysosome、microsome. It made lipide and protein of these membrance dissociate and degenerate, membrance penetration increased. Typical biochemical changes appeared, 180bp bases DNA fragmentation ladder pattern was observed in agar electrophoresis.

但PDT作用后出现线粒体肿胀，可能是因为PDT的主要靶部位是膜性细胞器如线粒体、胞质膜、内质网和溶酶体等，光化学反应产物使这些膜的脂类和蛋白质发生分解变性，膜通透性增高所致。

In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy.

根据本发明，通过促进大量hG－CSF融合蛋白分泌到周质中的相当简单的方法可以高纯度制备hG－CSF蛋白，所述方法不需要复杂的加工过程例如对于通过常规技术分离胞质中产生的作为不溶性包函体的hG－CSF所需的溶解和随后再折叠，因此，在开发用于抗癌治疗的补充药物的过程中可用hG－CSF蛋白广泛地作为活性成分。

RESULTS: After 24 hours of preinduction, cells changed from spindle to polygonal or irregular, with many short rod-shape processes and 2-3 nucleoli. Whirlpool shape growth disappeared, and was disordered. After 1-3 hours of induction, cells gradually became round, with significant cell contraction, strong refraction, bipolar or pluripolar processes connected each other into a net. After 5 hours of induction, grades one and two branches were detected.

结果：预诱导24 h，胞体由原来的长梭形变成多角形或不规则形，伸出多个短棒状突起，可见2~3个核仁，细胞间漩涡状生长趋势消失，排列无规律；诱导1~3 h，细胞逐渐变圆，胞质收缩明显，折光性增强，双级或多极的突起互相连接呈网状；诱导5 h，突起出现一、二级分支。

These abnormal changes mainly occurred at two stages:(1) at meiotic prophase, almost half of Peiai 64S microsporocytes exhibited aberration, with sparse free ribosomes, underdeveloped mitochondria and many swollen endoplasmic reticula.

这些变化主要发生在两个阶段：（1）减数分裂前期，约半数的小孢子母细胞的胞质出现异常，游离核糖体稀少，并具有不发育线粒体和大量泡状内质网。

The subunit translocates to the nucleus, where it activates transcription.

胞外结构的二聚体化引起跨膜结构域在膜内进行横向扩散，并启动与胞质结构域的接触。

The ultrastructure of crystal idioblasts changed during the maturing of leaves, the plastids were gradually degenerated while cytoplasm matrix was gradually dry.

叶片成熟过程中，结晶细胞超微结构发生变化，质体逐渐退化，胞基质逐渐干缩。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。