胞质体

In contrast with the rapid nucleus degradation, cell organelles in cytoplasm, such as rough endoplasmic reticulum, amyloplast, and mitochondrion, maintained their metabolic functions for a longer time.

在核衰退的同时，其胞质中的粗面内质网、淀粉质体和线粒体等细胞器具有正常的代谢功能，细胞仍在合成并积累营养物质，淀粉胚乳细胞一边衰退一边行使其功能，直至死亡。

In the process of nucleus degeneration, cell organella, for example, rough endoplasmic reticulum, amyloplast, and mitochondrion, maintained normally metabolic functions in cytoplasm, and nutrient substance was still synthesized and accumulated at the time of nuclear degeneration, the starchy endosperm cells carried out their functions until cell death simultaneously.

在核衰退的同时，其胞质中的粗面内质网、淀粉质体和线粒体等细胞器具有正常的代谢功能，细胞仍在合成并积累营养物质，淀粉胚乳细胞表现出一边衰退一边在行使其功能，直到细胞死亡。

2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果：电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀，主要分布于神经元的胞浆中或突起内，神经元分化的初期，溶酶体集中分布于胞质内，随突起的长出，可见在突起内出现。

During the eighth to tenth month, the number of NOS positive cells was cut down obviously, but the cell bodies developed big with more cytoplasm and heavy dyeing.

第8~10个月龄时，胃腺中NOS阳性细胞数目显著减少，但细胞胞体大，胞质多，深染。

As a result penicillium of Ma Erni humble is double photograph bacterium, mould bacterium colony can discover the broom shape mycelial of diagnostic sex,; of pigment of rose of water-solubility of the generation after sanded Paul fosters 3 days is medullary inside and outside of cell of blood of the week outside mixing all can discover spore;PAS coloring sees bacterium body show circle, elliptic or sausage shape, size is differ, it is 2~8 μ M about, color of afterbirth wall incarnadine and clear and successive, it is thus clear that inside the cell of sausage shape one apparent horizontal stroke is lain between, afterbirth is qualitative not easy and chromatic.

结果马尔尼菲青霉菌为双相菌，霉菌型菌落可发现特征性的扫帚状菌丝，沙保罗培养3天后产生水溶性玫瑰色素；骨髓和外周血细胞内外均可发现孢子；PAS染色可见菌体呈圆形、椭圆形或腊肠状，大小不一，约为2~8μm，胞壁染红色且清楚连续，在腊肠状的细胞内可见一明显的横隔，胞质不易着色。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuo le membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中，质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物，线粒体和小泡上只有少量酶活性反应产物。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuole membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中，质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物，线粒体和小泡上只有少量酶活性反应产物。

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置，且被一团原生质所包围的，其余的游离核位于边缘的现象以及花粉管进入雌配子体前，合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型，有单萌发孔，外壁表面具小刺状纹饰，小刺基部宽大，末端尖或钝圆。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中，DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F，以重组质粒DNA和脂质体共转染COS-7细胞，用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。