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Prokaryotes lack organelles such as nucleoli, mitochondria, plastid s, Golgi apparatus, and do not exhibit cyclosis.

原核生物无细胞器,如核仁、线粒体、质体、高尔基体等,不出现胞质环流。

Taking self-fruitful rate as the criterion and segregating population of field survey as method, we got the result that the ratio of fertile plants and sterile plants in the three F2 population of K-cytoplasmic male sterile hybrid accords with 63:1 tested by Chi-square, therefore we can infer that the restorative mechanism of K-cytoplasmic male sterile line was controlled by three different major genes, which manifested as sporophore sterility.

结果表明:在三个K型胞质雄性不育的F2群体中,可育单株与不育单株的比例经卡方检验符合63:1的理论比例,因此可以推断小麦K型胞质雄性不育的恢复由3对主效恢复基因控制,并表现为孢子体不育类型。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

The recombinant proteins were purified according to the protocol of Ni-NTA Agarose and detected by SDS-PAGE, and TLC. Western blotting was used to identify their immunoreactivities with the serum of patient with clonorchiasis.

寄生性蠕虫以胞质GSTs为主,参与对由虫体代谢和宿主免疫系统所产生的脂质过氧化物和细胞毒性的羰基的解毒,对虫体的生存起重要的作用,是免疫疗法、化学疗法和免疫诊断潜在的靶标。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化:染色质固缩边聚,形成致密、均质的染色质块,胞质浓缩、电子密度升高并空泡化;凋亡巨噬细胞溶酶体、线粒体增多;细胞突起缩短减少,但巨噬细胞特有的板状伪足不消失。

Antoni type A areas consist of compact spindle-shaped cells with twisted nuclei and indistinct cytoplasmic borders. They are arranged in interlacing fascicles with nuclear palisading, whirling, and Verocay bodies.

Antoni A型区的细胞呈梭形,胞核扭曲,胞质边界模糊,细胞排列为呈束状交错或旋涡状,胞核呈栅栏状,有Verocay小体。

After the cells dealt with GS-Rh2 of 13.0 mg/L, the typical apoptosis cells were discovered: showing disorganized, highly contracted and vacuolated, condensed cytoplasm, vanished karyotheca, ruptured cell nucleus and apoptotic body.

凋亡细胞吉姆萨染色检测:经13.0 mg/L人参皂甙单体Rh2处理后,HL60细胞出现核染色质浓缩、呈周边凝聚,核膜裂解形成凋亡小体、胞质出现空泡但胞膜完整的典型细胞凋亡形态学改变。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果:与对照组相比,在6小时和8小时实验组中,可见成团的鹅卵石状室管细胞隆起于表面,微绒毛更为丰富,微绒毛根部可见到若干微细小孔,在SFO体部的一些室管膜细胞的胞膜完全破裂,胞质几乎荡然无存,仅剩胞核居于细胞的一侧,直径有5.7μm,呈分裂叶状;与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

Results: Embryoid bodies grew well in Collagen Gel, the differentiated cells grew into the gel gradually; electronmicroscope showed that some induced cells were rich in Golgi's bodies and endoplasmic reticulums, a few secretory granules were also visible, which was similar to human adult thyrocyte.

结果 拟胚体在鼠尾胶原上生长状态良好,分化细胞贴壁后逐渐陷入鼠尾胶原;电镜下诱导细胞高尔基体、内质网丰富,部分诱导细胞胞质内见不典型分泌颗粒,与人成体甲状腺细胞超微结构类似。

Blood-brain barrier in tumors and peritumoral oedematous regions had the same variations in ultrastructure.

瘤体和瘤周水肿区血脑屏障超微结构变化无明显差异,内皮肿胀,毛细血管腔狭小,胶面有稀疏的绒毛样突起,内皮为无孔型,胞质内胞饮泡较多,内皮紧密连接增长,细胞连接间隙增宽,基膜完整,胶质膜缺损。

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