胞质体

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

After treatment with AT9810,the cell became smallerand condensed,typical signs,such as cytoplasmicconcentration,chromatin agglutination,pyknotic necleus,necleus cleavage,apoptosis corpuscle,was found inmorphological observation.

经AT9810处理后的细胞体积变小皱缩，形态学观察具有典型凋亡细胞的特征：胞浆浓缩，染色质凝集，核固缩、裂解及凋亡小体形成。

Results In the normal control brains, 14-3-3 immunoreactivity was localized mainly in the neuronal somata and processes, and some glial cells showed only weak immunoreactivity.

结果 在正常脑组织标本中，13-3-3蛋白主要表达于神经元的胞体和突起，仅在少数的胶质细胞中可见14-3-3蛋白的弱表达。

In curarized, artificially ventilated rats, microinjection of sodium glutamate into nucleus arcuatus induced both depressor and bradycardiaresponse. On the contrary, injection of apomorphine into AR or Glu into substantianigra produced pressor and tachycardia effects.

本工作用神经元胞体兴奋剂L-谷氨酸钠注入箭毒化、人工呼吸大鼠的下丘脑弓状核，引起血压下降，心率减慢；多巴胺受体激动剂去水吗啡注入AR和Glu注入黑质均产生升压和加速心率的效应，Glui。

HCRT potentiates NMDAR-mediated somatic release from LC neuronsApplication of 100μM NMDA evoked a pronounced secretion.This response to NMDA was subsequently enhanced by treatment with 100 nM HCRT for 5 min.

HCRT增强LC神经元NMDA受体介导的胞体递质分泌给予100μM NMDA可以显著引起分泌信号，随后用100 nM HCRT处理5 min，再次给予100μM NMDA可引起更大的分泌信号(增加大约40%)。

A transposon mutagenesis system applicable for Xanthomonas campestrispv.campestrishas been developed by transferring the transposonTn5gusA5,which contains the gus reporter gene,to the broad host rangvector pLAFR1 and taking the advantage of incompatibility between pLAFR1and pPH1JI plasmids.Five different types of exopolysaccharidedeficient mutants related respectively to eight different mutated sites wereisolated by applying the system to mutagenize the Xcc wild type strain.

本研究将含gus报告基因的转座子Tn5gusA5转座到广谱宿主载体质粒pLAFR1后，利用pLAFR1与pPH1JI质粒的不相容性，建立了一套适合于甘蓝黑腐病菌的转座子诱变体系，应用该体系诱变甘蓝黑腐病菌野生型菌株，成功地分离到5种不同类型、分别涉及8个不同突变位点的胞外多糖突变体。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。