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The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP (hydrogen-peroxide oxidoreductase)逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数(sciatic nerve function index,SFI)测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况,探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用,为外周神经损伤的治疗提供新的理论基础。

RESULTS: Fifty minutes after addition of inducing medium, under the optical microscope, BMSCs exhibited a typical morphology of perikaryon. Three hours later, most of cells became into bipolar or multipolar neuron-like cell appearance with cell bodies and processes.

结果:加入诱导液50 min后,光镜下骨髓间充质干细胞呈典型的核周体形态,3 h后大多数细胞转变为双极或多极神经元细胞样形态,出现胞体和突起。

The CGRP-positive fibers in the MrD of the striatum connected dorsally with the bed nucleus of the stria terminals through the stria terminals, ventrally with the amygdaloid nucleus, and caudally with the CGRP-positive cell bodies in the lateral part of the substantia nigra and its dorsolateral area.(4) The CGRP-positive fibers connected mainly with the central amygdaloid nucleus, and less with the medial amygdaloid nucleus; the CGRP-positive fibers in the lateral border of the caudate putamen connected mainly with the central amygdaloid nucleus as well.

并与边缘区的CGRP阳性纤维带之间存在较多的横向联系纤维;(3)纹状体边缘区的CGRP阳性纤维向背侧通过终纹与吻侧的终纹床核联系,向腹侧与杏仁核的CGRP阳性纤维相连,向尾侧和中脑黑质外侧部及其背外侧区的CGRP阳性胞体相连;(4)边缘区的CGRP阳性纤维主要与杏仁中央核相联系,少量与杏仁内侧核相联系;尾壳核外侧缘的CGRP阳性纤维也主要与杏仁中央核相联系。

The type of cell death caused by ricin was related to the ricin concentration and time of incubation with HeLa cells. The typical apoptosis was induced by ricin 0.05μmol〓. The apoptotic cells mainly showed cell shrinkage, cytoplasmic membrane blebbing, the chromatin condensation and fragmentation, crescentic nuclear and membrane bound apoptotic bodies formation.

细胞死亡方式也与蓖麻毒素作用时间和浓度相关,0.05μmol.L〓的蓖麻毒素可引起HeLa细胞发生典型的细胞凋亡,主要特征是细胞体积缩小,胞浆起泡,细胞核染色质浓缩、凝集,新月状核和膜包裹核染色质的凋亡小体形成。

The large granulocytes show characteristics of holocrine, and the small hyalinocytes are a transient type of residual cell bodies of large granulocytes after the holocrine process finished.

大颗粒细胞还具有全质分泌的特性,小透明细胞极可能是大颗粒细胞全质分泌后的一时性残余胞体。

Tumor mass could express CD133 and nestin. The karyoplasmic ratio of CD133+ brain glioma stem cells reached 2/3-3/4, and there were a few processes, a few organelles and a plenty of ribosome. These were accorded with the characteristics of stem cells.

肿瘤球能同时表达干细胞标志物CD133和巢蛋白,CD133+脑胶质瘤干细胞核浆比例达2/3~3/4,突起少,胞浆中线粒体等细胞器较少,核糖体丰富,未见胶质丝等分化结构,符合干细胞超微结构特点。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤(10,15 gkg-1d-1)治疗后,异位内膜有较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小,核固缩,胞浆和核染色质凝集,密度增高,细胞间凋亡小体,同时间质细胞中可见一些坏死细胞;异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

PRL-LIR positive perikarya were mainly seen in nucleus suprachiasmaticus, nucleus paraventricularis preopticus, nucleus supraopticus, nucleus hypothalamus paraventricularis, nucleus periventricularis arcuatus and nucleus accumbens. PRL-LIR fibers and their terminals were detected in paleostriatum and median eminence.

结果,PRL阳性胞体主要分布在视交叉上核、视前室旁核、视上核、下丘脑室旁核、弓状核和伏隔核,旧纹状体、正中隆起存在大量阳性纤维末梢,在侧脑室腹侧的室管膜和脑基底神经胶质板上也存在PRL阳性神经元。

It has been proved by previous studies that peripheral nerve injury can induce a series of changes of expression of some neural active material and their receptors either in the somata or in the terminals of the damaged primary afferent neurons and motoneurons. These changes may reflect a re-ordering of metabolic priorities so that the synthesis of trophic factors or growth-associated proteins augmented while that of neurotransmitter-related proteins decreased.

以往的研究表明,周围神经损伤能够引起受损神经元(包括初级传入神经元和运动神经元)的胞体或终末内某些神经活性物质及其受体的表达发生一系列变化,其中与神经生长和营养有关的物质常发生上调,而与神经传递有关的物质则发生下调;与此同时,受损神经元的周围常伴随发生胶质反应及神经胶质增生等变化。

Transport signal in a soluble cargo protein (for example, a secreted protein that passes through the lumen) is a region that binds to the lumenal domain of a transmembrane cargo receptor, which in turn has an cytoplasmic domain that binds an adaptor protein. Interaction between the cargo and the coat

我们已经有了几种类型的信号的细节:要求一种构像用于蛋白质通过胞吞作用成为细胞内的一部分;一种氨基酸序列使得蛋白质到达内质网;和一种修饰使得蛋白质到达溶酶体(小的膜体,在那里蛋白质降解,见后面)。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。