胞质

Effects of Croton tiglium total alkaloids on cancer cell cytoplasm membrane fluidity and structure of the cytoplasmic matrix .

巴豆总生物碱对癌细胞质膜流动性及胞浆基质结构的影响。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

OBJECTIVE: To investigate transfection and expression of liposome-mediated cytosine deaminase gene in rabbit BMSCs.

目的：观察脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其表达。

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

RESULTS: ADMSCs at passage 3 were positive for CD13, CD59 and CD44, but negative for CD34 and HLA-DR. Chromosomal morphous, structure and number were normal, showing diplont structure. Under the microscope, there were complete Golgi complex, plenty of rough endoplasmic reticulum, and some glycogen granules in cytoplasm.

结果：第3代脂肪间充质干细胞CD13，CD44，CD59均呈阳性表达，而造血干细胞表面标志CD34及成纤维细胞表面标志HLA-DR均呈阴性表达；染色体的形态、结构和数目均未见异常，呈二倍体结构；镜下可见完整的高尔基复合体，数量较多的粗面内质网，部分糖元颗粒分布在胞浆中。

Fruit a berry, with mucilaginous pulp or dry, without pulp vesicles; endocarp membranous.

果为浆果，具黏质果肉或干燥，没有汁胞；内果皮膜质。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

During the secondary wall formation of fiber, H+-ATPase, Ca2+-ATPase and APase also sustainablely remained in plasma membrane, plasma membrane invagination, plasmodesmata, transfer vesicles and agglutinated nucleus.

在次生壁形成的整个过程中，ATP酶、Ca2+-ATPase和酸性磷酸酶在运输小泡、细胞膜、质膜内陷、胞间连丝和凝聚的染色质中将持续存在。

Four weeks later, in the baicalin group, some spindle-shaped UCB-MSCs began to present shrinkage, with slender processes on the cell edge, and some UCB-MSCs tended to be spherical-, conical-, and triangle-shaped appearance, with many slender processes on the pseudopodia.

细胞形态学观察：脐血单个核细胞培养第2~3天开始贴壁生长，2周左右达高峰，3周后细胞生长达80%~90%融合，此时脐血原始间充质干细胞呈较均一的长梭形。4周后，黄芩苷组原来呈梭形的胞体一部分发生收缩，细胞边缘出现细的突起；一部分胞体逐渐近似圆形、锥形、三角形，伪足有多个细长突起。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。