胞质

The effect of the specific inhibitor cibacron blue of GSTs and antiparasitic drugs albendazole, praziquantel and artesunate on the activities of recombinant GSTM, GST1 and GST2 was evaluated.

研究目的解析三个胞质GSTs的基因结构及其特点，克隆三个胞质GSTs基因，鉴定原核表达的重组GSTs的免疫反应性、免疫原性、催化活性及其免疫定位和抑制剂、常用抗寄生虫药对催化活性的影响。

By using the GFP-labeling technique , we have found that GFP-CaM colocalized with all the characterized structures formed during cytokinesis.

CaM在胞质分裂期不同阶段与不同特征结构的共分布提示CaM参与了胞质分裂的整个过程。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

488Anti-rat CD106 and Bis-Benzimide staining to calculate cytoplast purity. Mitochondrial membrane potential was detected by flow cytometry following labeling with Rhodamine123. RESULTS: The purity of cytoplasts was up to 95%.

488anti-rat CD106及Bis- Benzimide染色进行形态学观察，计算胞质体纯度；应用Rhodamine123标记后用流式细胞仪检测胞质体中线粒体膜势能。

RESULTS: Compared with control group, the activity of the LDH/ACP/Arg in the AMФ or PMФ and in the culture fluid of the AMФ or PMФ were significantly increased (P.05 or P.01), and the ability devouring neutral red and stimulating index in the PMФ were strengthened (P.01), the ergastoplasm was hyperplasia and the amount of lysosome was manifold in the spleen macrophages of old rats induced by paecilomyces cicadidae total polysaccharides.

结果：蝉拟青霉总多糖使老龄大鼠肺泡巨噬细胞、腹腔巨噬细胞胞内和细胞培养上清液内ACP、LDH、arginase活力显著高于对照组（P.05或P.01）；使PMФ的吞噬功能及其刺激指数显著高于对照组（均P.01）；使老龄大鼠脾脏细胞胞质粗面内质网和溶酶体数量多于对照组。

Under the light microscope, we found that transfected SGC7901 gastric cancer cells with NHE1 antisense gene were larger with abundant cytoplasm and comparatively same size of nuclei, and the ratio of nuclei to cytoplasm, mitotic count and heteromorphism decreased when they were compared with their parent cells.

与亲本细胞相比，光镜下NHE1反义基因转染的SGC7901胃癌细胞胞体增大，胞质丰富，核大小相对一致，核质比和核分裂象减少，异型性减少。

W7 was added when the cells entered anaphase, we found that although some cells had elongated, the distribution patternof CaM at central spindle was normal, and it seemed that a cleavage furrow would possibly form soon, however, these cells stopped cytokinesis at this stage, no furrow ingression followed.

本实验中我们还观察到在胞质分裂后期，CaM与Y一微管蛋白共分布于中体两端。W7处理同步化在胞质分裂后期的细胞，不但使中体解聚发生延迟，而且抑制了Y一微管蛋白与中体的解离。

It is suggested that the algae symbiot can affect host organellar function,quantity and location, stimulate the food uptake and digestive activity, restrain the functional activity of the organelle, change the gene expression and cause the change of genome structiure in the P. bursaria-chlorellae system.

据结果推测，纤毛虫中的藻类共生体可影响宿主细胞中胞器的功能、数量和分布，可影响细胞核核仁的数量和分布；可影响宿主细胞的形态、生长、抗氧化能力及胞质糖含量；可影响宿主细胞的食物摄取及相关胞器的消化活性，抑制宿主胞器的功能活动；并可改变宿主细胞基因的表达，导致细胞基因组结构的变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。