胞质

Result In the HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT, expressions of hTERT, Bcl-2 and mitochondrial cyt C were significantly down-regulated, while Bax and cytoplastic cyt C were obviously up-regulated, and active caspase-9 was found in addition to procaspase-9 compared with that in negative control cells and untransfected cells.

结果 与未转染细胞和对照细胞相比，转染细胞hTERT、Bcl-2和线粒体cyt C表达明显减少，Bax和胞质cyt C表达明显增加；未转染细胞及对照细胞仅见约46kD的前caspase-9条带，转染细胞可见46kD前caspase-9条带和35kD caspase-9 p35亚基条带。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱，采用Western blot检测线粒体，胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

Then histochemical, electron microscopic, enzyme histo cytochemical and confocal laser scanning microscopic methods were employed to study the distribution of vinculin, cathepsin Dand nematolysosomes ...

结果在正常对照组神经元，组织蛋白酶D与纽蛋白分布于胞质及突起内；在CD及PMA处理神经元，纽蛋白及组织蛋白酶D的分布呈向心性移动，但集聚的部位不同；电镜酶细胞化学方法显示CD组及PMA组神经元内线状溶酶体均增多。

Meanwhile, we found the colocalization of mRabL5 with WGA-positive endosome by immunofluorescence assay, which further demonstrated that mRabL5 may cycle between cytolsol and membrane system to facilitate endocytosis pathway.

利用该抗体，我们检测到mRabL5是一个广谱表达的蛋白，与TGN共定位，作为一种小G蛋白，循环于膜系统和胞质之间，参与内吞途径。

Large amount of vital cells were obtained by using this method. Cultured hepatocytes are epithelioid cells and have various shape.

培养的乳鼠肝细胞呈上皮状，形态多样，可分为大形细胞和小形细胞，胞质内充满颗粒。

PART 2:The molecular mechanisms of Nontypeable Haemophilus influenzae induced inflammatory response in respiratory epithelial cells Objective:Nontypeable Haemophilus influenzaewas traditionally considered as an extracellular pathogen in respiratory infections.In this study,we established a model of infected cells to confirm NTHI was also a facultatively intracellular pathogen.Mitogen activated protein kinasesare the important signal transduction pathway in mediating inflammatory response induced by NTHI in respiratory epithelium.In this study,we elucidate the impact of NTHI,and its SCF and EP on the expression of MAPK and subsequent proinflammatory mediators.

第二部分：NTHI致呼吸道上皮细胞炎症反应的分子机制研究目的：通过建立呼吸道上皮细胞株的体外NTHI感染模型，确认NTHI在细胞内的存活，观察NTHI及其组分(可溶性胞质成分SCF、外膜蛋白EP)对呼吸道上皮细胞MAPK信号传导通路和后续的前炎症因子表达的影响，并应用特异性ERK-MAPK抑制剂U0126、p38 MAPK抑制剂SB203580特异性阻断下游MAPK信号传导通路，观察它们对呼吸道上皮细胞分泌前炎症因子表达的影响。

Results By using zinc-iodide-osmium staining, the tubulo-vesicular system of parietal cells of fundic gland was manifested as numerous fibrous structures under HVEM. The nematolysosomes was often demonstrated as branching in shape and anastomosed with each other making a network system in cytoplasm under the visual field of the three dimensional electron microscopy combined with AcPase cytochemistry.

结果高压电镜下显示，锌-碘-锇酸染色方法制备的标本胃底腺壁细胞的微管泡系统呈无数纤维状结构；AcPase结合三维电子显微镜观察显示，线性线粒体在胞质中常呈分支状并互相吻合成网。

Observation of cultured cells stained by haematoxylin and eosin: It was shown that the cells presented round or ellipse. There was little cytoplasm, presenting acidophily. Nucleus was big and round, single in the center, deep stained.

培养细胞苏木精-伊红染色观察结果：可见细胞圆形或椭圆形，胞质少呈嗜酸性，细胞核大而圆，单个居中，着色深。

However, Ooplasmic transfer can lead to mtDNA become heteroplastic, which is the mtDNA of donor and recipient coexist in offspring.

但是，卵胞质转移也可能会导致线粒体DNA异质，即供体和受体的线粒体DNA同时存在于后代体内。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。